The Probiotic Bacterium Isolated from Bekasam (Traditional Fermented Food), . Induces Activation of Gut Mucosal Immune System in Rat.

BACKGROUND

Bekasam is one of the traditional foods in South Sumatra, Indonesia, a mixture of fermented fish containing Lactic Acid Bacteria (LAB), . Non-commensal bacteria and probiotics can induce intestinal mucosal immune responses.

AIM

This pilot study aimed to see the efficacy of . to the immune response of the intestinal mucosa by assessing the levels of IgA in the intestinal fluid and markers of T cell populations, such as CD4 and CD8 in the intestinal mucosa.

METHODS

This study was an in vivo experimental study. As many as 30 rats were grouped into 3 treatment groups (doses 107, 108, and 109 CFU/rat/day, for 7 days) and 2 groups of controls (negative control, 10% non-fat milk, and positive control, 108 CFU/rat/day for 7 days). At the end of the treatment, the intestinal mucosa was taken to examine the levels of IgA, CD4 and CD8 using the Enzyme-Linked Immunosorbent Assay (ELISA) method, according to the manuals of each ELISA kit. All displays of research data were presented with means ± SD. T-test was used to assess the significance of differences.

RESULTS

Secretion of Ig A increased with the addition of . from bekasam. Administration of . yielded no effect on helper T cell level (CD4 markers), as well as on cytotoxic T cell levels (CD8 markers).

CONCLUSION

. probiotic from bekasam improved the intestinal mucosal immune system by increasing the production of Ig A, but exhibited no effect on T lymphocyte cells.