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马来西亚白蜘蛛百合(和)叶枯病病原菌的鉴定与特性分析

Identification and Characterization of Causing Leaf Blight on White Spider Lilies ( and ) in Malaysia.

作者信息

Huda-Shakirah Abd Rahim, Kee Yee Jia, Hafifi Abu Bakar Mohd, Mohamad Azni Nurul Nadiah, Zakaria Latiffah, Mohd Masratul Hawa

机构信息

School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia.

出版信息

Mycobiology. 2019 Nov 4;47(4):408-414. doi: 10.1080/12298093.2019.1682448. eCollection 2019.

Abstract

and , commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015-2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on and . Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-α) gene, the causal pathogen was identified as . Phylogenetic analysis of combined dataset of ITS and TEF1-α grouped the isolates studied with other isolates of from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts.

摘要

并且,通常被称为蜘蛛百合,是球根多年生草本植物,在马来西亚作为观赏植物广泛种植。在2015 - 2016年期间,在槟城几个地点的寄主上发现了叶枯病症状。症状表现为不规则的褐色至微红病斑,周围有黄色晕圈。随着病情发展,受感染的叶片枯萎、干枯并脱落,在病斑部位出现黑色微菌核和分生孢子器。本研究旨在调查蜘蛛百合叶枯病的致病病原菌。基于形态特征以及内部转录间隔区(ITS)区域和翻译延伸因子1 - α(TEF1 - α)基因的DNA序列,致病病原菌被鉴定为[病原菌名称未给出]。对ITS和TEF1 - α联合数据集的系统发育分析将所研究的分离株与来自GenBank的其他[病原菌名称未给出]分离株归为一组。分离株的分组得到了96%的自展值支持。致病性测试证明了该真菌在引起两种寄主叶枯病中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b203/6968666/1e9b6b83497e/TMYB_A_1682448_F0001_C.jpg

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