Chen Yanqiu, Sun Xiaobo, Pan Wei, Yu Guifeng, Wang Jinping
College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, China.
Front Chem. 2020 Jan 15;7:911. doi: 10.3389/fchem.2019.00911. eCollection 2019.
In this article, the Fe-sensitive carbon dots were obtained by means of a microwave-assisted method using glutamic acid and ethylenediamine as reactants. The carbon dots exhibited selective response to Fe ions in aqueous solution with a turn-off mode, and a good linear relationship was found between (F-F)/F and the concentration of Fe in the range of 8-80 μM. As a result, the as-synthesized carbon dots can be developed as a fluorescent chemosensor for Fe in aqueous solution. Moreover, the carbon dots can be applied as a fluorescent agent for fungal bioimaging since the fungal cells stained by the carbon dots were brightly illuminated on a confocal microscopy excited at 405 nm. Furthermore, an increase in the concentration of intracellular Fe could result in fluorescence quenching of the carbon dots in the fungal cells when incubated in the Tris-HCl buffer solution containing Fe. However, due to EDTA might hinder Fe(III) to enter the fungal cells, incubation in Fe(III)-EDTA complex solution exerted negligible effect on the fluorescence of fungal cells labeled by the carbon dots. Therefore, the carbon dots can serve as a potential probe for intracellular imaging of Fe inside fungal cells.
在本文中，以谷氨酸和乙二胺为反应物，通过微波辅助法制备了对铁敏感的碳点。该碳点在水溶液中对铁离子呈现出关闭模式的选择性响应，并且在8 - 80 μM范围内，(F - F)/F与铁浓度之间存在良好的线性关系。因此，所合成的碳点可被开发为水溶液中铁的荧光化学传感器。此外，由于在405 nm激发的共聚焦显微镜下，被碳点染色的真菌细胞能被明亮地照亮，所以该碳点可作为真菌生物成像的荧光剂。此外，当在含有铁的Tris - HCl缓冲溶液中孵育时，细胞内铁浓度的增加会导致真菌细胞中碳点的荧光猝灭。然而，由于EDTA可能会阻碍Fe(III)进入真菌细胞，在Fe(III)-EDTA复合溶液中孵育对被碳点标记的真菌细胞的荧光影响可忽略不计。因此，碳点可作为真菌细胞内铁的细胞内成像的潜在探针。
