Hou Pengchong, Zhang Xian, Lu Qian, Chen Shunwei, Liu Qiang, Qiao Congde, Zhao Hui
School of Materials Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
Polymers (Basel). 2022 Jul 27;14(15):3045. doi: 10.3390/polym14153045.
In this study, four polycarbazole derivatives (PCMB-Ds) with different alkyl side chains were designed and synthesized via Wittig-Horner reaction. A novel solid-phase electrochemiluminescence (ECL) system was prepared by immobilizing PCMB-D on an indium tin oxide (ITO) electrode with polyvinylidene fluoride (PVDF) in the presence of tripropylamine (TPrA). It could be found that the increase in alkyl side chain length had little effect on the ECL signal of PCMB-D, while the increase in the degree of polymerization (DP) greatly enhanced the ECL signal. Furthermore, the P-3/ITO ECL sensor based on the polyoctylcarbazole derivative (P-3) with the best ECL performance was successfully constructed and detected Fe under the optimal experimental conditions. The ECL signal steadily diminished with the increased concentration of Fe because of the competition and complexation between Fe and P-3 under the condition of pH 7.4. This P-3/ITO platform could realize a highly sensitive and selective detection of Fe with a wide detection range (from 6 × 10 mol/L to 1 × 10 mol/L) and low detection limit of 2 × 10 mol/L, which could allow the detection of Fe in multiple scenarios, and would have a great application prospect.
在本研究中，通过维蒂希-霍纳反应设计并合成了四种具有不同烷基侧链的聚咔唑衍生物（PCMB-Ds）。通过在三丙胺（TPrA）存在下用聚偏二氟乙烯（PVDF）将PCMB-D固定在氧化铟锡（ITO）电极上，制备了一种新型的固相电化学发光（ECL）体系。可以发现，烷基侧链长度的增加对PCMB-D的ECL信号影响不大，而聚合度（DP）的增加则大大增强了ECL信号。此外，成功构建了基于具有最佳ECL性能的聚辛基咔唑衍生物（P-3）的P-3/ITO ECL传感器，并在最佳实验条件下检测了铁。在pH 7.4的条件下，由于铁与P-3之间的竞争和络合作用，随着铁浓度的增加，ECL信号稳步减弱。该P-3/ITO平台能够实现对铁的高灵敏度和选择性检测，检测范围宽（从6×10⁻⁹mol/L到1×10⁻⁵mol/L），检测限低至2×10⁻⁹mol/L，可用于多种场景下铁的检测，具有广阔的应用前景。
