Gao Jie, Ma Baowei, Lu Yun, Zhang Yifeng, Tong Yuru, Guo Siyuan, Gao Wei, Huang Luqi
School of Traditional Chinese Medicine, School of Pharmaceutical Sciences, and Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing 100069, China.
State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China.
ACS Omega. 2020 Jan 13;5(3):1414-1421. doi: 10.1021/acsomega.9b02919. eCollection 2020 Jan 28.
Flavonoid glycosides have shown many pharmacological activities in clinical studies. However, the main way to obtain flavonoid glycosides is to extract and separate them from plants, which wastes both time and resources. Here, we identified the -glycosyltransferase (UGTs) TwUGT3 from and analyzed its bioinformatics. First, the enzyme was found to utilize phloretin and uridine diphosphate glucose (UDPG) as substrates to produce an acid-tolerant glucoside. Then, it also can use quercetin and UDPG as substrates to produce the corresponding -glucoside. In addition, we further explored the substrate specificity of TwUGT3, which suggested that it also accepts luteolin, pinocembrin, and genistein to produce the corresponding -glucosides. Subsequently, the optimum pH, reaction time, reaction temperature, and enzymatic kinetic parameters of TwUGT3 were determined.
黄酮苷在临床研究中已显示出许多药理活性。然而，获得黄酮苷的主要方法是从植物中提取和分离它们，这既浪费时间又浪费资源。在此，我们从[具体来源]中鉴定出β-糖基转移酶（UGTs）TwUGT3并分析了其生物信息学。首先，发现该酶利用根皮素和尿苷二磷酸葡萄糖（UDPG）作为底物来产生一种耐酸糖苷。然后，它也可以使用槲皮素和UDPG作为底物来产生相应的β-葡萄糖苷。此外，我们进一步探索了TwUGT3的底物特异性，结果表明它还接受木犀草素、松属素和染料木黄酮来产生相应的β-葡萄糖苷。随后，确定了TwUGT3的最佳pH、反应时间、反应温度和酶动力学参数。
