Lu Yun, Ma Bao-Wei, Gao Jie, Tu Li-Chan, Hu Tian-Yuan, Zhou Jia-Wei, Liu Yuan, Tu Yu-He, Lin Zhao-Shou, Huang Lu-Qi, Gao Wei
School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
Yong'an State Owned Forest Farm, Yong'an 366000, China.
J Asian Nat Prod Res. 2020 Jun;22(6):537-546. doi: 10.1080/10286020.2019.1642330. Epub 2019 Jul 24.
Flavonoids are important secondary metabolites that exist in many medicinal plants. Flavonoid glycosyltransferases can transfer sugar moieties to their parent rings, producing various flavonoid glycosides with significant pharmacological activities. Here, we report the molecular cloning of the O-glycosyltransferase TwUGT2 from and its catalytic activity was explored by heterologous expression in . The results showed that TwUGT2 has specific glycosyltransferase activity towards C-3 and 7 hydroxyl groups of flavonoids, thereby converting quercetin and pinocembrin into isoquercitrin and pinocembrin 7-O-beta-D-glucoside, respectively. The identification of TwUGT2 will provide a useful molecular tool for synthetic biology and contribute to drug discovery.[Formula: see text].
黄酮类化合物是存在于许多药用植物中的重要次生代谢产物。黄酮类糖基转移酶可以将糖基部分转移到其母环上，产生具有显著药理活性的各种黄酮苷。在此，我们报道了来自[具体来源未给出]的O-糖基转移酶TwUGT2的分子克隆，并通过在[具体宿主未给出]中的异源表达探索了其催化活性。结果表明，TwUGT2对黄酮类化合物的C-3和7羟基具有特定的糖基转移酶活性，从而分别将槲皮素和松属素转化为异槲皮苷和松属素7-O-β-D-葡萄糖苷。TwUGT2的鉴定将为合成生物学提供一个有用的分子工具，并有助于药物发现。[公式：见原文]
