胶体金免疫层析法快速检测鸡胸肉中的卡巴多司和喹乙醇。
Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast.
作者信息
Guo Lingling, Wu Xiaoling, Cui Gang, Song Shanshan, Kuang Hua, Xu Chuanlai
机构信息
State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
出版信息
ACS Omega. 2020 Jan 13;5(3):1422-1429. doi: 10.1021/acsomega.9b02931. eCollection 2020 Jan 28.
Abused or misused carbadox (CBX) and cyadox (CYA) in animal feed may cause food safety concerns, threatening human health. Here, we describe the design of a novel hapten for preparation of a monoclonal antibody against CBX and CYA simultaneously. Using this antibody, colloidal gold immunochromatographic assay (GICA) was developed for screening of CBX and CYA residues in chicken breast. Under optimal conditions, semiquantitative analysis results were visible by eye, with a visual limit of detection of 8 μg/kg for CBX and CYA, and cut-off values of 20 μg/kg for CBX and 40 μg/kg for CYA in chicken breast. Quantitative analysis could be performed using a hand-held strip scanner, with a calculated limit of detection of 2.92 μg/kg for CBX and 2.68 μg/kg for CYA in chicken breast. Validated by liquid chromatography-MS/MS, the developed GICA provides a useful tool for rapid on-site CBX and CYA residue screening in chicken breast.
在动物饲料中滥用或误用卡巴多司（CBX）和喹乙醇（CYA）可能引发食品安全问题，威胁人类健康。在此，我们描述了一种新型半抗原的设计，用于同时制备抗CBX和CYA的单克隆抗体。利用该抗体，开发了胶体金免疫层析法（GICA）用于检测鸡胸肉中CBX和CYA残留。在最佳条件下，半定量分析结果肉眼可见，CBX和CYA的可视检测限为8 μg/kg，鸡胸肉中CBX的临界值为20 μg/kg，CYA的临界值为40 μg/kg。可使用手持式试纸扫描仪进行定量分析，鸡胸肉中CBX的计算检测限为2.92 μg/kg，CYA的计算检测限为2.68 μg/kg。经液相色谱-质谱/质谱验证，所开发的GICA为鸡胸肉中CBX和CYA残留的快速现场筛查提供了一种有用的工具。
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