College of Plant Protection, Gansu Agricultural University, Lanzhou, China.
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
J Appl Microbiol. 2020 Aug;129(2):256-265. doi: 10.1111/jam.14601. Epub 2020 Feb 21.
In this study, the antifungal effect of cinnamaldehyde against Fusarium sambucinum and its underlying mechanisms were determined.
Minimum inhibitory concentration and minimal fungicidal concentration of cinnamaldehyde were 3 and 4 mmol l on spore germination and colony development assays in vitro, respectively. Furthermore, the lesion diameter of potato tubers and tuber slices inoculated with F. sambucinum was reduced by 76·9 and 69% after treatment with 4 mmol l cinnamaldehyde. Cytometric analyses revelled that cinnamaldehyde significantly affected the integrity of cell membrane firstly, then decreased mitochondrial membrane potential and induced the accumulation of intracellular reactive oxygen species. Meanwhile, high-performance liquid chromatography results indicated that 3 mmol l cinnamaldehyde could reduce the ergosterol content by 67·94%. This effect was accompanied by a down-regulation of ERG11, ERG6 and ERG4 which were involved in ergosterol biosynthesis.
Theses results suggest that cinnamaldehyde exerts strong antifungal activity against F. sambucinum, probably by affecting the ergosterol biosynthetic processes what leads to the disruption of cell membrane integrity.
Cinnamaldehyde is a predominant constituent and key flavour compound of cinnamon essential oil. It has been used as a food additive and flavorant. It is expected to be a novel and safe fungicide for controlling dry rot in potato tubes.
本研究旨在确定肉桂醛对桑枝镰孢菌的抗真菌作用及其作用机制。
体外孢子萌发和菌落发育试验中，肉桂醛的最小抑菌浓度和最小杀菌浓度分别为 3 和 4 mmol·l-1。此外，用 4 mmol·l-1肉桂醛处理后，马铃薯块茎和切片上桑枝镰孢菌的病斑直径分别减少了 76.9%和 69%。细胞计量分析表明，肉桂醛首先显著影响细胞膜的完整性，然后降低线粒体膜电位并诱导细胞内活性氧的积累。同时，高效液相色谱结果表明，3 mmol·l-1肉桂醛可使麦角固醇含量降低 67.94%。这种作用伴随着与麦角固醇生物合成有关的 ERG11、ERG6 和 ERG4 的下调。
这些结果表明，肉桂醛对桑枝镰孢菌具有很强的抗真菌活性，可能通过影响麦角固醇生物合成过程导致细胞膜完整性破坏。
肉桂醛是肉桂精油的主要成分和关键风味化合物。它已被用作食品添加剂和香料。它有望成为一种新型、安全的防治马铃薯块茎干腐病的杀菌剂。
