Wang Jianglai, Zhang Jinfeng, Ma Jinxiu, Liu Lu, Li Jiajia, Shen Tong, Tian Yongqiang
School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, China.
Research Institute, Lanzhou Jiaotong University, Lanzhou, China.
J Appl Microbiol. 2022 Apr;132(4):3125-3141. doi: 10.1111/jam.15458. Epub 2022 Feb 2.
Root rot caused by Fusarium solani is an important disease seriously affecting the yield and quality of Astragalus membranaceus. Therefore, this study was performed to elucidate the antifungal activities and mechanisms of cinnamaldehyde treatment against F. solani and its control effect for A. membranaceus root rot.
Cinnamaldehyde significantly inhibited mycelial growth and spore germination of F. solani in dose-dependent, and the median effective concentration was 178.68 μl l . Furthermore, scanning electron microscopy, propidium iodide staining, cell leakage experiments and ergosterol quantitation illuminated that cinnamaldehyde could alter the mycelial morphology, damage the plasma membrane and hinder the biosynthesis of ergosterol. Besides, cinnamaldehyde induced the generation of reactive oxygen species by synergistically upregulating the genes encoded subunits for NADPH oxidase. The disease suppression efficacy of 600 μl l cinnamaldehyde against A. membranaceus root rot was 92.98 ± 6.08% (p < 0.05) under greenhouse conditions.
This study proved that cinnamaldehyde could markedly inhibit the growth of F. solani in vitro and effectively suppress the occurrence of A. membranaceus root rot, perhaps by inducing oxidative damage, which results in the distortion of F. solani, and the destruction of cell membrane integrity and permeability.
This study first explores the antifungal mechanisms of cinnamaldehyde against F. solani in vivo and vitro, thereby providing a promising candidate for disease biocontrol.
茄腐镰刀菌引起的根腐病是严重影响黄芪产量和品质的重要病害。因此，本研究旨在阐明肉桂醛处理对茄腐镰刀菌的抗真菌活性及作用机制，以及对黄芪根腐病的防治效果。
肉桂醛对茄腐镰刀菌的菌丝生长和孢子萌发具有显著的剂量依赖性抑制作用，半数有效浓度为178.68 μl/l。此外，扫描电子显微镜、碘化丙啶染色、细胞渗漏实验和麦角甾醇定量分析表明，肉桂醛可改变菌丝形态，损伤细胞膜并阻碍麦角甾醇的生物合成。此外，肉桂醛通过协同上调NADPH氧化酶编码亚基的基因诱导活性氧的产生。在温室条件下，600 μl/l肉桂醛对黄芪根腐病的防治效果为92.98±6.08%（p<0.05）。
本研究证明，肉桂醛可能通过诱导氧化损伤，导致茄腐镰刀菌变形以及细胞膜完整性和通透性的破坏，从而在体外显著抑制茄腐镰刀菌的生长，并有效抑制黄芪根腐病的发生。
本研究首次探索了肉桂醛在体内外对茄腐镰刀菌的抗真菌机制，从而为病害生物防治提供了一个有前景的候选物。
