Advanced Technology Center for Aging Research, Scientific Technological Area, IRCCS INRCA, Ancona, Italy.
Institute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Ancona, Italy.
Nat Prod Res. 2021 Nov;35(22):4779-4784. doi: 10.1080/14786419.2020.1721729. Epub 2020 Feb 3.
Gram-negative sepsis ranks as the leading cause of death in intensive care units. Despite the development of new antibiotics, mortality from gram-negative sepsis remains high. The present study aims to investigate the effects of berberine (BBR) administration on septic death induced by intraperitoneal injection. The results showed that (i) single 5 mg/kg dose of BBR increases the survival of septic mice, (ii) BBR administration improves the antimicrobial efficacy of antibiotic drug, (iii) BBR pre-treatment prevents improvements of BBR therapy without affecting the pro-survival effects of antibiotic drug. The effects of BBR administration were associated with immunological alterations represented by changes in CD4+ and CD8+ lymphocytes population and IL-6 and TNF-α production. This study highlighted the benefits of berberine administration as antibiotic adjuvant in sepsis. Furthermore, information about berberine-induced immunological perturbations and their influence on host response to infection and therapy has been shown.
革兰氏阴性菌败血症是重症监护病房死亡的主要原因。尽管新型抗生素不断发展，革兰氏阴性菌败血症的死亡率仍然很高。本研究旨在探讨小檗碱（BBR）给药对内毒素血症小鼠死亡的影响。结果表明：（i）单次 5mg/kg 的 BBR 剂量可增加败血症小鼠的存活率；（ii）BBR 给药可提高抗生素药物的抗菌效果；（iii）BBR 预处理可防止 BBR 治疗的改善，而不影响抗生素药物的生存效果。BBR 给药的效果与免疫改变有关，表现为 CD4+和 CD8+淋巴细胞群以及 IL-6 和 TNF-α的产生变化。本研究强调了小檗碱作为败血症抗生素佐剂的应用价值。此外，还展示了有关小檗碱诱导的免疫扰动及其对宿主感染和治疗反应的影响的信息。
