Li Ying, Cao Zhe-Tong, Wang Xin-Yue, Ge Xi-Zhen
a Beijing Key Laboratory of Biomass Waste Resource Utilization , Biochemical Engineering College, Beijing Union University , Beijing 100023 , China.
J Asian Nat Prod Res. 2018 Apr;20(4):374-384. doi: 10.1080/10286020.2017.1384818. Epub 2017 Sep 28.
Berberine (BBR) is a traditional Chinese medicine in various applications due to its antibacterial effect. Here we investigated the increased bacterial resistance of E. coli toward BBR. The median effective concentration (EC) of BBR against E. coli was increased when TetA efflux protein (TEP) was introduced. Sixty-five percent of the intracellular BBR was expelled and molecular docking demonstrated the intensive interaction of TEP to BBR. Finally, the combined antibacterial experiment identified that BBR acted as an inhibitor of TEP in detoxification of tetracycline. TEP is the first discovered protein that was related to the bacterial susceptibility to BBR.
黄连素（BBR）因其抗菌作用而在多种应用中作为一种中药。在此，我们研究了大肠杆菌对BBR的耐药性增加情况。当引入四环素外排蛋白（TEP）时，BBR对大肠杆菌的半数有效浓度（EC）增加。65%的细胞内BBR被排出，分子对接表明TEP与BBR有强烈相互作用。最后，联合抗菌实验确定BBR在四环素解毒过程中作为TEP的抑制剂。TEP是首个被发现与细菌对BBR敏感性相关的蛋白。
