Gongadze M, Machavariani M, Enukidze M, Gogia N, Iobadze M, Chkhikvishvili I
Tbilisi State Medical University, Institute of Medical Biotechnology, Georgia.
Georgian Med News. 2019 Dec(297):154-157.
The aim of our study is to evaluate the mechanisms of anti-inflammatory property of Tagetes patula L. (French Marigold) flower extracts and its protective effect on renal epithelium against uropathogenic E. coli infection. Thus, in this study, a number of in vitro assays were performed to evaluate the antioxidant and anti-inflammatory activities of Tagetes patula L flower extract (water soluble part of French Marigold flower, extracted with chloroform). MTT assay was performed to determine the effect of French Marigold flower extract on cell viability in MDCK cells activated with 100ng/mL of LPS. We investigated the effect of Tagetes patula L. extract on LPS-stimulated levels of NO and TNF-α production by Griess reaction based colorimetric assay and ELISA respectively. Also, we analyzed content of the French Marigold flower extract by HPLC. Our results demonstrated that TPE protected MDCK cells and increased their viability. Also, TPE had a strong inhibitory effect on NO secretion. These effects were due to inhibition of TNF-α induced pro-apoptotic pathway. The Tagetes patula L extract represents a rich source of lutein and its antioxidant property can be caused by lutein. Our results suggest that TPE could be developed as an anti-oxidant and anti-inflammatory agent derived from natural products.
我们研究的目的是评估孔雀草（法国万寿菊）花提取物的抗炎特性机制及其对肾上皮细胞抵抗尿路致病性大肠杆菌感染的保护作用。因此，在本研究中，进行了多项体外试验以评估孔雀草花提取物（法国万寿菊花的水溶性部分，用氯仿提取）的抗氧化和抗炎活性。采用MTT法测定法国万寿菊花提取物对用100ng/mL LPS激活的MDCK细胞活力的影响。我们分别通过基于Griess反应的比色法和ELISA法研究了孔雀草提取物对LPS刺激的NO和TNF-α产生水平的影响。此外，我们通过HPLC分析了法国万寿菊花提取物的含量。我们的结果表明，TPE保护MDCK细胞并提高其活力。此外，TPE对NO分泌有强烈的抑制作用。这些作用是由于抑制了TNF-α诱导的促凋亡途径。孔雀草提取物是叶黄素的丰富来源，其抗氧化特性可能由叶黄素引起。我们的结果表明，TPE可开发成为一种源自天然产物的抗氧化和抗炎剂。
