LMU University of Munich , Department of Chemistry , Butenandtstr. 13 , D-81377 Munich , Germany.
J Phys Chem A. 2020 Feb 27;124(8):1554-1560. doi: 10.1021/acs.jpca.9b11225. Epub 2020 Feb 14.
A dyad of orthogonally arranged chromophores of benzoperylenehexacarboxylictrisimide (energy donor, D) and terrylenetetracarboxylic bisimide (energy acceptor, A) was synthesized where a quantitative energy transfer (FRET) from D to A was found, although not only are both transition moments orthogonally arranged but the spectral overlap between the fluorescence of the energy donor and the absorption of the acceptor is also small. Even an appreciable further diminishing of the overlap in a dyad with phenylbenzoxazole as the donor could not inhibit the energy transfer. Thus, the application of the FRET theory to real systems is estimated to be limited where molecular vibrations seem to play a key role in the energy transfer.
苯并菲六羧酸三异亚胺（能量供体，D）和均苯四羧酸二酰亚胺（能量受体，A）的一对正交排列的发色团被合成，其中发现了从 D 到 A 的定量能量转移（FRET），尽管不仅两个跃迁矩是正交排列的，而且能量供体的荧光与受体的吸收之间的光谱重叠也很小。即使在以苯并恶唑为供体的偶联物中进一步显著减小重叠，也不能抑制能量转移。因此，FRET 理论在实际系统中的应用估计是有限的，其中分子振动似乎在能量转移中起着关键作用。
