Department of Thoracic Surgery, Shaanxi People's Hospital, Xi'an, China.
Department of Pathology, Xi'an Fourth Hospital, Xi'an, China.
Clin Exp Pharmacol Physiol. 2020 Jul;47(7):1283-1290. doi: 10.1111/1440-1681.13269. Epub 2020 Feb 17.
Esophageal squamous cell carcinoma (ESCC) is a major type of esophageal cancer, accounting for about 90% of cases. Circular RNA UBAP2 (circUBAP2) is involved in the progression of several types of cancers. However, the role of circUBAP2 in ESCC remains unclear. In the present study, circUBAP2 expression was found to be upregulated in ESCC tumour tissues. Knockdown of circUBAP2 through infection with lentiviral vector encoding shRNA targeting circUBAP2 (sh-circUBAP2) inhibited the proliferation, migration and invasion of ESCC cells. In addition, circUBAP2 significantly promoted the proliferation, migration and invasion of ESCC cells. In vivo xenograft assay demonstrated that circUBAP2 downregulation suppressed the tumour growth of ESCC. Further mechanism investigations proved that circUBAP2 exerted its role via sponging microRNA (miR)-422a, and miR-422a directly targeted Rab10 in ESCC cells. These findings suggested that circUBAP2 acted as oncogene through regulating the miR-422a/Rab10 axis in ESCC.
食管鳞状细胞癌（ESCC）是一种主要的食管癌类型，约占病例的 90%。环状 RNA UBAP2（circUBAP2）参与了几种类型癌症的进展。然而，circUBAP2 在 ESCC 中的作用尚不清楚。在本研究中，发现 ESCC 肿瘤组织中 circUBAP2 的表达上调。通过感染靶向 circUBAP2 的慢病毒载体（sh-circUBAP2）抑制 circUBAP2 的表达，可抑制 ESCC 细胞的增殖、迁移和侵袭。此外，circUBAP2 显著促进 ESCC 细胞的增殖、迁移和侵袭。体内异种移植实验表明，circUBAP2 的下调抑制了 ESCC 的肿瘤生长。进一步的机制研究证明，circUBAP2 通过海绵吸附 microRNA（miR）-422a 发挥作用，而 miR-422a 可直接靶向 ESCC 细胞中的 Rab10。这些发现表明，circUBAP2 通过调节 ESCC 中的 miR-422a/Rab10 轴发挥癌基因作用。
