环状 RNA circADAMTS6 促进 ESCC 进展并与预后相关。
The circRNA circADAMTS6 promotes progression of ESCC and correlates with prognosis.
机构信息
Department of Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.
Tumor Research Institute, The Fourth Hospital of Hebei Medical University, 050017, Shijiazhuang, Hebei, People's Republic of China.
出版信息
Sci Rep. 2022 Aug 12;12(1):13757. doi: 10.1038/s41598-022-17450-2.
Circular RNAs (circRNAs) are a type of noncoding RNA, which play a vital role in the occurrence and development of esophageal squamous cell carcinoma (ESCC). While the role of novel circADAMTS6 in ESCC remains unknown. We assessed circADAMTS6 expression in ESCC tissues and cells, and the relationship between circADAMTS6 expression and overall survival of ESCC patients. Functional experiments in vitro and xenograft in vivo assay were applied to explore the functions and mechanisms of circADAMTS6 in ESCC. Results found that up-regulation of circADAMTS6 was associated with poor overall survival and may acted as an independent risk factor for ESCC prognosis. Knockdown of circADAMTS6 significantly inhibited the proliferation, migration and invasion of ESCC cells and growth of xenograft tumors in vivo. Induced AGR2 expression was able to rescue the loss of function induced by si-circADAMTS6 in KYSE150 cell. CircADAMTS6 may acts as oncogene by activating AGR2 and the Hippo signaling pathway coactivator YAP in ESCC.
环状 RNA（circRNAs）是一种非编码 RNA，在食管鳞状细胞癌（ESCC）的发生和发展中起着至关重要的作用。虽然新型 circADAMTS6 在 ESCC 中的作用尚不清楚。我们评估了 ESCC 组织和细胞中 circADAMTS6 的表达，以及 circADAMTS6 表达与 ESCC 患者总生存率之间的关系。体外功能实验和体内异种移植实验用于探讨 circADAMTS6 在 ESCC 中的功能和机制。结果发现，circADAMTS6 的上调与总生存率差相关，可能是 ESCC 预后的独立危险因素。circADAMTS6 的敲低显著抑制了 ESCC 细胞的增殖、迁移和侵袭以及体内异种移植肿瘤的生长。诱导 AGR2 表达能够挽救 si-circADAMTS6 在 KYSE150 细胞中引起的功能丧失。circADAMTS6 可能通过激活 ESCC 中的 AGR2 和 Hippo 信号通路共激活因子 YAP 发挥癌基因作用。
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