解析：这是一篇标题为“从生孢噬纤维菌中鉴定一种自足细胞色素 P450 单加氧酶用于对映选择性苄基羟化”的学术论文的标题。 因此，这段标题的译文为： 从生孢噬纤维菌中鉴定一种自足细胞色素 P450 单加氧酶用于对映选择性苄基羟化
Characterization of a Self-Sufficient Cytochrome P450 Monooxygenase from Deinococcus apachensis for Enantioselective Benzylic Hydroxylation.
机构信息
Key Laboratory of Biocatalysis & Chiral Drug Synthesis of Guizhou Province, Generic Drug Research Center of Guizhou Province, Green Pharmaceuticals Engineering Research Center of Guizhou Province, School of Pharmacy, Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563000, China.
出版信息
Chembiochem. 2020 Jul 1;21(13):1820-1825. doi: 10.1002/cbic.201900691. Epub 2020 Feb 27.
A self-sufficient cytochrome P450 monooxygenase from Deinococcus apachensis (P450DA) was identified and successfully overexpressed in Escherichia coli BL21(DE3). P450DA would be a member of the CYP102D subfamily and assigned as CYP102D2 according to the phylogenetic tree and sequence alignment. Purification and characterization of the recombinant P450DA indicated both NADH and NADPH could be used by P450DA as a reducing cofactor. The recombinant E. coli (P450DA) strain was functionally active, showing excellent enantioselectivity for benzylic hydroxylation of methyl 2-phenylacetate. Further substrate scope studies revealed that P450DA is able to catalyze benzylic hydroxylation of a variety of compounds, affording the corresponding chiral benzylic alcohols in 86-99 % ee and 130-1020 total turnover numbers.
从生胞菌（Deinococcus apachensis）中鉴定出一种自给自足的细胞色素 P450 单加氧酶（P450DA），并在大肠杆菌 BL21（DE3）中成功过表达。P450DA 是 CYP102D 亚家族的成员，并根据系统发育树和序列比对被分配为 CYP102D2。重组 P450DA 的纯化和表征表明，P450DA 可以使用 NADH 和 NADPH 作为还原辅因子。重组大肠杆菌（P450DA）菌株具有功能性，对甲基 2-苯基乙酸酯的苄位羟化表现出优异的对映选择性。进一步的底物范围研究表明，P450DA 能够催化各种化合物的苄位羟化，以 86-99%ee 和 130-1020 总周转数的方式获得相应的手性苄醇。
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