Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
Chembiochem. 2020 Jun 2;21(11):1567-1572. doi: 10.1002/cbic.201900781. Epub 2020 Mar 3.
Pradimicins (PRMs) constitute an exceptional class of natural products that show Ca -dependent recognition of d-mannose (Man). In addition to therapeutic uses as antifungal drugs, the application of PRMs as lectin mimics for glycobiological research has been attracting considerable interest, since the emerging biological roles of Man-containing glycans have been highlighted. However, only a few attempts have been made to use PRMs for glycobiological purposes. The limited use of PRMs is primarily due to the early assumption that the readily modifiable carboxyl group of PRMs is involved in Ca binding, and thus, not available to prepare research tools. Recently, this assumption has been disproved by structural elucidation of the Ca complex of PRMs, which paves the way for designing carboxyl group modified derivatives of PRMs for research use. This article outlines studies related to Ca -mediated Man binding of PRMs and discusses their application for glycobiology.
普拉地霉素（PRMs）是一类具有特殊结构的天然产物，能够依赖钙离子识别 D-甘露糖（Man）。除了作为抗真菌药物的治疗用途外，PRMs 作为凝集素模拟物在糖生物学研究中的应用也引起了相当大的关注，因为含 Man 的聚糖的新兴生物学作用已被强调。然而，仅进行了一些将 PRMs 用于糖生物学目的的尝试。PRMs 的使用受限主要是因为早期假设 PRMs 中易于修饰的羧基参与了 Ca 结合，因此无法用于制备研究工具。最近，通过 PRMs 的 Ca 配合物的结构阐明否定了这一假设，为设计用于研究的 PRMs 羧基修饰衍生物铺平了道路。本文概述了与 PRMs 的 Ca 介导的 Man 结合相关的研究，并讨论了它们在糖生物学中的应用。
