Institute for Glyco-core Research (iGCORE), Nagoya University.
RIKEN Cluster for Pioneering Research.
Proc Jpn Acad Ser B Phys Biol Sci. 2022;98(1):15-29. doi: 10.2183/pjab.98.002.
Pradimicins (PRMs) are an exceptional family of natural products that specifically bind d-mannose (Man). In the past decade, their scientific significance has increased greatly, with the emergence of biological roles of Man-containing glycans. However, research into the use of PRMs has been severely limited by their inherent tendency to form water-insoluble aggregates. Recently, we have established a derivatization strategy to suppress PRM aggregation, providing an opportunity for practical application of PRMs in glycobiological research. This article first outlines the challenges in studying Man-binding mechanisms and structural modifications of PRMs, and then describes our approach to address them. We also present our recent attempts toward the development of PRM-based research tools.
普拉地米辛（PRMs）是一类特殊的天然产物家族，它们专门与 d-甘露糖（Man）结合。在过去的十年中，随着含 Man 聚糖的生物学作用的出现，它们的科学意义大大增加。然而，PRMs 的使用研究受到其固有形成水不溶性聚集的倾向的严重限制。最近，我们建立了一种衍生化策略来抑制 PRM 聚集，为 PRMs 在糖生物学研究中的实际应用提供了机会。本文首先概述了研究 Man 结合机制和 PRMs 结构修饰的挑战，然后描述了我们解决这些挑战的方法。我们还介绍了我们最近在开发基于 PRM 的研究工具方面的尝试。
