Somasundaran M, Robinson H L
Department of Pathology, University of Massachusetts Medical Center, Worcester 01655.
Science. 1988 Dec 16;242(4885):1554-7. doi: 10.1126/science.3201245.
The expression of a laboratory strain of HIV-1 (HTLV-IIIB) has been studied in mitogen-stimulated peripheral blood lymphocytes (PBLs) and in two lymphoid cell lines (CEM cells and C8166 cells). HIV-expressing cells contained from 300,000 to 2,500,000 copies of viral RNA per cell. Near-synchronous expression of an active infection could be achieved in C8166 cells. In these cells, the high copy numbers of viral RNA used as much as 40% of total protein synthesis for the production of viral gag protein, with high levels of viral RNA and protein synthesis preceding cell death by 2 to 4 days.
已在丝裂原刺激的外周血淋巴细胞（PBL）以及两种淋巴样细胞系（CEM细胞和C8166细胞）中研究了实验室株HIV-1（HTLV-IIIB）的表达情况。表达HIV的细胞每个细胞含有300,000至2,500,000拷贝的病毒RNA。在C8166细胞中可实现活跃感染的近同步表达。在这些细胞中，高拷贝数的病毒RNA在病毒gag蛋白产生过程中消耗了多达40%的总蛋白质合成，高水平的病毒RNA和蛋白质合成先于细胞死亡2至4天出现。
