Vestri A, Margheri G, Landini E, Meacci E, Tiribilli B
Molecular Biology Research Unit, Department of Experimental and Clinical Biomedical Sciences Mario Serio, University of Florence, Viale GB Morgagni 50, 50134 Firenze, Italy.
Institute of Complex Systems ISC-CNR, Via Madonna del Piano 10, Sesto Fiorentino, 50019 Firenze, Italy.
Rev Sci Instrum. 2020 Jan 1;91(1):013106. doi: 10.1063/1.5111829.
The widespread diffusion of low-cost but high-performance hardware is enhancing the realization of scientific equipment with features at the research laboratory level. In this paper, we demonstrate hardware implementation of a surface plasmon resonance compact device with high accuracy and measurement times appropriate for many applications. Image acquisition is realized by a Raspberry Pi single board computer with a camera module, and a Python code is used to process data. A flexible optical setup can work in two different configurations, namely, the inspection mode and angle resolved measurement mode. The inspection mode is used to precisely locate the light-emitting diode interrogation beam on the sample, avoiding uneven or faulty regions. The measurement mode allows us to monitor in real time the position of the minimum reflectivity with subpixel resolution. Performance tests show a resolution in the bulk refractive index of 4.9 × 10 refractive index units for 10 s acquisition time.
低成本但高性能硬件的广泛普及正在推动具有研究实验室级功能的科学设备的实现。在本文中，我们展示了一种表面等离子体共振紧凑型设备的硬件实现，该设备具有高精度且测量时间适用于多种应用。图像采集由带有摄像头模块的树莓派单板计算机实现，并且使用Python代码来处理数据。灵活的光学设置可以在两种不同配置下工作，即检测模式和角度分辨测量模式。检测模式用于将发光二极管询问光束精确地定位在样品上，避免不均匀或有故障的区域。测量模式使我们能够以亚像素分辨率实时监测最小反射率的位置。性能测试表明，在10秒采集时间内，体折射率分辨率为4.9×10折射率单位。
