Rippa Massimo, Castagna Riccardo, Sagnelli Domenico, Vestri Ambra, Borriello Giorgia, Fusco Giovanna, Zhou Jun, Petti Lucia
Institute of Applied Sciences and Intelligent Systems "E. Caianiello" of CNR, 80078 Pozzuoli, Italy.
Istituto Zooprofilattico Sperimentale del Mezzogiorno (IZSM), 80055 Portici, Italy.
Nanomaterials (Basel). 2021 Mar 31;11(4):886. doi: 10.3390/nano11040886.
is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify in milk by our portable SERS device. Upon bacterial capture from samples (10 cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection.
是一种全球范围内影响经济和医疗保健的食源性病原体。表面增强拉曼光谱（SERS）纳米生物传感可能是检测它的一种有前景的策略。我们将用于拉曼增强的高性能准晶图案化纳米腔与共价固定的第比利斯噬菌体作为高性能生物受体相结合。我们将高效的SERS纳米生物传感器与拉曼系统耦合，以开发一个能够监测目标细菌的基于噬菌体的现场生物传感平台。所开发的生物传感器使我们能够通过便携式SERS设备在牛奶中识别。从样品（10个细胞）中捕获细菌后，观察到与病原体识别相关的信号，证明了我们的系统在现场和食品检测中的具体适用性。
