Department of Biochemistry, BioFrontiers Institute , University of Colorado Boulder , Boulder , Colorado 80309 , United States.
ACS Chem Neurosci. 2020 Feb 19;11(4):508-514. doi: 10.1021/acschemneuro.0c00006. Epub 2020 Feb 6.
Ionic Zn has increasingly been recognized as an important neurotransmitter and signaling ion in glutamatergic neuron pathways. Intracellular Zn transiently increases as a result of neuronal excitation, and this Zn signal is essential for neuron plasticity, but the source and regulation of the signal is still unclear. In this study, we rigorously quantified Zn, Ca, and pH dynamics in dissociated mouse hippocampal neurons stimulated with bath application of high KCl or glutamate. While both stimulation methods yielded Zn signals, Ca influx, and acidification, glutamate stimulation induced more sustained high intracellular Ca and a larger increase in intracellular Zn. However, the stimulation-induced pH change was similar between conditions, indicating that a different cellular change is responsible for the stimulation-dependent difference in Zn signal. This work provides the first robust quantification of Zn dynamics in neurons using different methods of stimulation.
离子态锌（Ionic Zn）越来越被认为是谷氨酸能神经元通路中一种重要的神经递质和信号离子。由于神经元兴奋，细胞内锌会短暂增加，而这种锌信号对于神经元可塑性至关重要，但信号的来源和调节仍不清楚。在这项研究中，我们严格定量分析了用高浓度氯化钾或谷氨酸浴液刺激分离的小鼠海马神经元时，细胞内锌、钙和 pH 的动态变化。虽然这两种刺激方法都产生了锌信号、钙内流和酸化，但谷氨酸刺激诱导了更持续的高细胞内钙和更大的细胞内锌增加。然而，刺激诱导的 pH 变化在两种条件下相似，表明不同的细胞变化导致了刺激依赖性的锌信号差异。这项工作首次使用不同的刺激方法对神经元中的锌动态进行了稳健的定量分析。
