College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education , Shandong Normal University , Jinan 250014 , China.
CAS Key Laboratory of Coastal Environment Processes and Ecological Remediation, The Research Center for Coastal Environment Engineering and Technology, Yantai Institute of Coastal Zone Research , Chinese Academy of Sciences , Yantai 264003 , China.
ACS Sens. 2020 Feb 28;5(2):464-473. doi: 10.1021/acssensors.9b02205. Epub 2020 Feb 18.
In this paper, we first proposed a novel hybrid three-dimensional (3D) printed and paper-based microfluidic platform and applied it for investigating the cell's apoptosis and intracellular cross-talk. The fabrication of a 3D-printed microfluidic chip is much easier than polydimethylsiloxane (PDMS) chip and can be applied in many common labs without soft lithogrophy fabrication equipment. Moreover, 3D printing can be perfectly combined with paper-based chips that can provide 3D scaffold for cell culture and analysis. In addition, these paper chips are disposable after use, greatly reducing the experimental cost. We integrated "Christmas Tree" structure with the top layer of the 3D-printed microfluidic chip to generate a continuous concentration gradient, and the bottom layer contained paper-based chips as cell culture area. The two-layer structure allows the concentration gradient forming layer to be separated from the cell culture layer, which can simplify the planting of cells in the microfluidic chip and make sure the cells stay in the culture chambers and don't clog the microfluidic channels. Applying this hybrid platform, we examined the effect of HS on cancer cells. Continuous exposure to a low concentration of HS inhibited cancer cell SMMC-7721 proliferation by inducing cell apoptosis. We also found that two gaseous molecules HS and NO have cross-talk in cancer cells; they formed bioactive intermediate polysulfides in cancer cells. It is expected that this novel hybrid 3D-printed and paper-based microfluidic platform will have widespread application prospects in cell investigation.
在本文中，我们首先提出了一种新颖的混合三维（3D）打印和纸质微流控平台，并将其应用于研究细胞凋亡和细胞内串扰。3D 打印微流控芯片的制造比聚二甲基硅氧烷（PDMS）芯片更容易，并且可以在许多没有软光刻制造设备的普通实验室中应用。此外，3D 打印可以与纸质芯片完美结合，为细胞培养和分析提供 3D 支架。此外，这些纸质芯片在使用后是一次性的，大大降低了实验成本。我们将“圣诞树”结构与 3D 打印微流控芯片的顶层集成在一起，以产生连续的浓度梯度，而底层包含纸质芯片作为细胞培养区。这种两层结构允许浓度梯度形成层与细胞培养层分离，这可以简化微流控芯片中细胞的种植，并确保细胞留在培养室内，不会堵塞微流道。应用这种混合平台，我们研究了 HS 对癌细胞的影响。持续暴露于低浓度 HS 会通过诱导细胞凋亡来抑制癌细胞 SMMC-7721 的增殖。我们还发现两种气态分子 HS 和 NO 在癌细胞中有串扰；它们在癌细胞中形成了生物活性的中间多硫化物。预计这种新颖的混合 3D 打印和纸质微流控平台将在细胞研究中具有广泛的应用前景。
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