Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Universidade Estadual "Júlio de Mesquita Filho", Jaboticabal, Brazil.
Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Universidade Estadual "Júlio de Mesquita Filho", Jaboticabal, Brazil; Laboratório de Ciências Ambientais e Sustentabilidade Agropecuária, Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil; Laboratório de Ecologia de Mamíferos, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Ticks Tick Borne Dis. 2020 May;11(3):101401. doi: 10.1016/j.ttbdis.2020.101401. Epub 2020 Jan 30.
Even though Hepatozoon spp. has been molecularly detected in several wild animals in Brazil, there is no report on the occurrence of Hepatozoon spp. DNA in bats in Brazil. This study aimed at detecting Hepatozoon, in addition to ectoparasites, in non-hematophagous bats sampled in central-western Brazil using blood smears, hematoxylin-eosin (HE)-staining liver/spleen preparations and molecular and phylogenetic techniques. A total of 135 spleen, 127 liver, and 133 blood samples were collected from 135 non-hematophagous bats from 12 different species in two different sites in Campo Grande, Mato Grosso do Sul state, in the Brazilian Cerrado region. Spleen and blood DNA samples were submitted to two conventional PCR protocols for Hepatozoon spp. based on 18S rRNA. No Hepatozoon spp. gamonts or meronts were observed in blood smears and HE-stained-liver preparations, respectively. While none of the spleen samples was positive for Hepatozoon spp. in the PCR assays, 5 (3 %) blood samples contained 18S rRNA Hepatozoon DNA, including 2/37 (5 %) Artibeus lituratus, 2/32 (6 %) A. planirostris, and 1/23 (4 %) Platyrrhinus lineatus. Out of 5 bats positive for Hepatozoon spp., 3 were parasitized by either Macronyssidae/Spinturnicidae mites or Streblidae flies. BLAST analysis showed that the sequences detected in bats had >99 % identity with Hepatozoon sequences detected in amphibians and reptiles from Brazil, including Hepatozoon caimani detected in Caiman crocodilus. The phylogenetic inferences estimated by the Maximum Likelihood and Bayesian methods clustered the Hepatozoon sequences detected in Brazilian bats with those detected in reptiles and amphibians.
尽管在巴西的几种野生动物中已经通过分子手段检测到了 Hepatozoon spp.，但在巴西的蝙蝠中尚未有关于 Hepatozoon spp. DNA 发生的报道。本研究旨在通过血涂片、苏木精-伊红（HE）染色的肝/脾制剂以及分子和系统发育技术，检测巴西中西部非吸血蝙蝠中的 Hepatozoon 以及外寄生虫。共从巴西塞拉多地区南马托格罗索州坎波格兰德市的两个不同地点的 12 种不同蝙蝠中采集了 135 份脾、127 份肝和 133 份血样。从脾和血样中提取 DNA，通过基于 18S rRNA 的两种常规 PCR 方案对 Hepatozoon spp. 进行检测。在血涂片和 HE 染色的肝切片中均未观察到 Hepatozoon spp. 的裂殖体或配子体。虽然 Hepatozoon spp. 的 PCR 检测结果中脾样本均为阴性，但有 5 份（3%）血样含有 Hepatozoon 18S rRNA DNA，其中 2/37（5%）的 A. lituratus、2/32（6%）的 A. planirostris 和 1/23（4%）的 Platyrrhinus lineatus。在 Hepatozoon spp. 阳性的 5 只蝙蝠中，有 3 只感染了 Macronyssidae/Spinturnicidae 螨或 Streblidae 蝇。BLAST 分析表明，在蝙蝠中检测到的序列与巴西两栖动物和爬行动物中检测到的 Hepatozoon 序列具有>99%的同一性，包括在 Caiman crocodilus 中检测到的 Hepatozoon caimani。最大似然法和贝叶斯法的系统发育推断将在巴西蝙蝠中检测到的 Hepatozoon 序列聚类与在爬行动物和两栖动物中检测到的序列聚类在一起。
