de Rezende M B, Herrera H M, Carvalho C M E, Carvalho Anjos E A, Ramos C A N, de Araújo F R, Torres J M, de Oliveira C E
Universidade Católica Dom Bosco - UCDB, Campo Grande, MS, Brazil.
Bolsista CAPES - Proc. Number 1218-13-1, University of Manitoba, Winnipeg, Canada.
Transbound Emerg Dis. 2017 Dec;64(6):e36-e42. doi: 10.1111/tbed.12597. Epub 2017 Feb 24.
The multihost parasites Leishmania spp. infect a broad range of wild mammalian species including bats. Several species of bats have adapted to a variety of food resources and shelters in urban areas. This study aimed to detect Leishmania spp. DNA in bats present in forest fragments located in metropolitan areas endemic for leishmaniasis in Campo Grande, Mato Grosso do Sul (MS), Brazil. Blood samples were obtained from 80 individuals, including eight species of Phyllostomidae and one species of Vespertilionidae. Thirty of the 80 bats were positive for Leishmania spp. using conventional PCR, all belonging to the family Phyllostomidae. Eighteen samples tested by real-time PCR (qPCR) using specific primers for the kDNA of Leishmania infantum were positive. To the best of our knowledge, this is the first report detecting Leishmania spp. in Platyrrhinus incarum in addition to being the first reported detection of L. infantum in the bat species Phyllostomus discolor, Platyrrhinus lineatus, Artibeus planirostris and Artibeus lituratus. Our results show that bats can host Leishmania spp. in areas endemic for leishmaniasis, which must be taken into account in disease control operations by public health authorities.
多宿主寄生虫利什曼原虫属感染包括蝙蝠在内的多种野生哺乳动物。几种蝙蝠已经适应了城市地区的各种食物资源和栖息地。本研究旨在检测位于巴西南马托格罗索州大坎普市利什曼病流行的大都市地区森林碎片中的蝙蝠体内的利什曼原虫属DNA。从80只个体采集了血液样本，包括8种叶口蝠科蝙蝠和1种蝙蝠科蝙蝠。使用常规PCR检测，80只蝙蝠中有30只为利什曼原虫属阳性，均属于叶口蝠科。使用针对婴儿利什曼原虫kDNA的特异性引物通过实时PCR（qPCR）检测的18个样本呈阳性。据我们所知，这是首次在印加扁鼻蝠中检测到利什曼原虫属，也是首次在变色叶口蝠、条纹扁鼻蝠、平嘴犬吻蝠和花面犬吻蝠等蝙蝠物种中检测到婴儿利什曼原虫。我们的结果表明，在利什曼病流行地区，蝙蝠可以携带利什曼原虫属，公共卫生当局在疾病控制行动中必须考虑到这一点。
