Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science & Technology (UST), Daejeon 34113, Republic of Korea.
Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Bioresour Technol. 2020 Apr;302:122840. doi: 10.1016/j.biortech.2020.122840. Epub 2020 Jan 21.
Herein, a two-stage cultivation process was devised to overcome low pigment content of algal biomass grown in heterotrophy. Post-treatment conditions (i.e., light intensity, temperature, pH and salinity) were initially tested for dense heterotrophically-grown Chlorella sp. HS2 cultures in a multi-channel photobioreactor (mcPBR), and the results clearly indicated the influence of each abiotic factor on algal pigment production. Subsequently, the optimal post-treatment conditions (i.e., 455 μmol m s, 34.8℃, pH 8.23 and 0.7% (w/v) salinity), in which highest accumulation of algal pigments is expected, were identified using Response Surface Methodology (RSM). Compared to the control cultures grown in mixotrophy for the same duration of entire two-stage process, the results indicated a significantly higher pigment productivity (i.e., 167.5 mg L day) in a 5-L fermenter following the post-treatment at optimal conditions. Collectively, these results suggest that the post-treatment of heterotrophic cultures can be successfully deployed to harness the nascent algae-based bioeconomy.
在此，设计了两阶段培养工艺来克服异养生长的藻生物质中低色素含量的问题。首先针对多通道光生物反应器（mcPBR）中高密度异养生长的 Chlorella sp. HS2 培养物，对后处理条件（即光照强度、温度、pH 值和盐度）进行了测试，并明确指出了每种非生物因素对藻类色素产生的影响。随后，利用响应面法（RSM）确定了最佳后处理条件（即 455 μmol m s、34.8℃、pH8.23 和 0.7%（w/v）盐度），预计在此条件下藻类色素的积累最高。与在同一两段式工艺过程中以混合营养方式生长的对照培养物相比，在优化条件下进行后处理后，在 5-L 发酵罐中获得了显著更高的色素生产力（即 167.5 mg L day）。总体而言，这些结果表明，可以成功采用异养培养物的后处理来利用新兴的藻类生物经济。
