Department of Biological Systems Engineering, L.J. Smith Hall, Washington State University, Pullman, WA 99164-6120, USA.
Bioresour Technol. 2012 Jan;103(1):484-8. doi: 10.1016/j.biortech.2011.09.122. Epub 2011 Oct 5.
A two-stage heterotrophic and phototrophic culture strategy for algal biomass and lipid production was studied, wherein high density heterotrophic cultures of Chlorellasorokiniana serve as seed for subsequent phototrophic growth. The data showed growth rate, cell density and productivity of heterotrophic C.sorokiniana were 3.0, 3.3 and 7.4 times higher than phototrophic counterpart, respectively. Hetero- and phototrophic algal seeds had similar biomass/lipid production and fatty acid profile when inoculated into phototrophic culture system. To expand the application, food waste and wastewater were tested as feedstock for heterotrophic growth, and supported cell growth successfully. These results demonstrated the advantages of using heterotrophic algae cells as seeds for open algae culture system. Additionally, high inoculation rate of heterotrophic algal seed can be utilized as an effective method for contamination control. This two-stage heterotrophic phototrophic process is promising to provide a more efficient way for large scale production of algal biomass and biofuels.
采用两段式异养-光照培养策略来进行藻类生物质和油脂的生产，其中，高密度的异养小球藻培养液可作为后续光照培养的藻种。研究数据表明，与光照培养相比，异养小球藻的生长速率、细胞密度和生产力分别高出 3.0、3.3 和 7.4 倍。当将异养和光照藻种接种到光照培养系统中时，它们具有相似的生物质/油脂产量和脂肪酸组成。为了扩大应用范围，研究还测试了废水和食物垃圾作为异养生长的原料，结果表明这些原料可以成功支持细胞生长。这些结果表明，使用异养藻类细胞作为开放藻类培养系统的藻种具有优势。此外，高接种率的异养藻种可以作为一种有效的污染控制方法。这种两段式异养-光照的工艺有望为藻类生物质和生物燃料的大规模生产提供更有效的方法。
