State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
Sci Total Environ. 2020 May 1;715:136377. doi: 10.1016/j.scitotenv.2019.136377. Epub 2020 Jan 7.
The deammonification process is a promising and energy efficient nitrogen removal technology. Since deammonification process has succeeded in high-strength ammonia nitrogen wastewater treatment (sidestream deammonification) but its application in treating low-strength ammonium nitrogen wastewater (mainstream deammonification) remains a great challenge. In this study, mainstream deammonification process in two reactors maintained stability with hydrazine (NH) addition. The two reactors consisted of a deammonification granular reactor and a mixed ammonia oxidizing bacteria (AOB) flocculent with anaerobic ammonia oxidizing bacteria (AnAOB) granular reactor. Deammonification granular reactor had a more efficient total nitrogen removal efficiency (TNRE, 80.5 ± 5.8%) and nitrogen removal rate (NRR, 0.33 ± 0.04 g/(L·day)). The advantage of retain biomass in granular sludge reactor lead to a more balanced ex-situ activity between AOB (0.37 mg N/(g VSS·h)) and AnAOB (0.43 mg N/(g VSS·h)). Candidatus Brocadia and Nitraspira were detected the dominant genus responsible for the observed AnAOB and nitrite oxidizing bacteria (NOB), respectively. The more obvious effect of NH on enhancing AnAOB and suppressing NOB both in ex-situ activity and genus abundances in mixed sludge reactor were also founded may due to loose spatial distribution among species.
脱氨过程是一种有前途且节能的氮去除技术。由于脱氨过程已成功应用于高强度氨氮废水处理（侧流脱氨），但其在处理低强度铵氮废水（主流脱氨）方面仍面临巨大挑战。在本研究中，通过添加联氨（NH），使两个反应器中的主流脱氨过程保持稳定。这两个反应器由脱氨颗粒反应器和混合氨氧化菌（AOB）絮体与厌氧氨氧化菌（AnAOB）颗粒反应器组成。脱氨颗粒反应器具有更高的总氮去除效率（TNRE，80.5±5.8%）和氮去除率（NRR，0.33±0.04 g/（L·天））。颗粒污泥反应器中保留生物量的优势导致 AOB（0.37 mg N/（g VSS·h））和 AnAOB（0.43 mg N/（g VSS·h））之间的体外活性更加平衡。分别检测到 Candidatus Brocadia 和 Nitraspira 是负责观察到的 AnAOB 和亚硝酸盐氧化菌（NOB）的主要属。在混合污泥反应器中，也发现 NH 对增强 AnAOB 和抑制 NOB 的体外活性和属丰度的影响更为明显，这可能是由于物种之间的空间分布松散。
