State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Center for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
Bioresour Technol. 2021 Jul;331:125058. doi: 10.1016/j.biortech.2021.125058. Epub 2021 Mar 27.
How to achieve stable mainstream deammonification is still a huge challenge. In this work, satisfactory nitrogen removal were achieved in a deammonification granular sludge reactor (R1, 0.42 ± 0.03 kg N / (m·d)) and a mixed flocculent with granular sludge reactor (R2, 0.39 ± 0.04 kg N / (m·d)) at ambient temperature (21-28 ℃) . The good adaptability of anammox bacteria (Candidatus Jettenia) to ambient temperature ensured its efficient activity (0.84-1.54 mg N/(g VSS·h)). The overexpression ammonia monooxygenase gene abundances in ammonia oxidizing bacteria (Nitrosomonas) was also predicted. The inhibition of hydrazine and the competition of denitrifying bacteria (Denitratisoma) to nitrite nitrogen, leading to a low Nitrospira relative abundances (0.2%-2.1%) . It was also found that R1 was more resistant to the unfavorable condition. For R2, higher Denitratisoma abundances (9.2%-18.5%) and predicted metabolic pathway abundances related to carbon metabolism were observed.
实现稳定的主流脱氨仍然是一个巨大的挑战。在这项工作中，在常温（21-28℃）下，脱氨颗粒污泥反应器（R1，0.42±0.03 kg N/（m·d））和混合絮状颗粒污泥反应器（R2，0.39±0.04 kg N/（m·d））中实现了令人满意的氮去除效果。厌氧氨氧化菌（Candidatus Jettenia）对环境温度的良好适应性确保了其高效的活性（0.84-1.54 mg N/（g VSS·h））。还预测了氨氧化菌（Nitrosomonas）中氨单加氧酶基因的过度表达丰度。肼的抑制和反硝化菌（Denitratisoma）对亚硝酸盐氮的竞争，导致 Nitrospira 的相对丰度较低（0.2%-2.1%）。还发现 R1 对不利条件的抵抗力更强。对于 R2，观察到更高的 Denitratisoma 丰度（9.2%-18.5%）和预测与碳代谢相关的代谢途径丰度。
