European hares do not avoid newly pesticide-sprayed fields: Overspray as unnoticed pathway of pesticide exposure.

Although risk assessments for pesticides involve quantifying exposure routes for animals, little empirical evidence exists on how individuals use areas where pesticides were used. Further, the European Food Safety Authority guidelines for the risk assessment of birds and mammals currently only include direct dietary uptake from exposed plants as important pathway for pesticides, arguing that dermal exposure is generally negligible. Here, we use the European hare (Lepus europaeus) as a model of a farmland specialist to investigate if (1) hares adjust habitat use in response to pesticide spraying, using GPS data, and (2) calculate the pesticide exposure and uptake over different pathways, i.e. foraging uptake versus overspray and subsequent oral grooming, using an agent-based modelling approach. Apart from avoidance of sprayed fields on the spraying day by inactive hares, which was likely caused by the mechanical disturbance rather than the pesticide itself, we found no evidence that hares reduced the use of pesticide-sprayed fields compared to control observations where no pesticides were applied. Our simulation showed that both the proportion of exposed individuals and the pesticide uptake were related to the pathway of exposure (foraging versus overspray/oral grooming), and depended on the age class and the vegetation type. We found that pesticide uptake via overspray/oral grooming might be 7-fold higher compared to foraging and might thus be an important pathway of pesticide exposure in hares. Our findings emphasize that policy makers, specifically the European Food Safety Authority, should incorporate alternative pathways of pesticide exposure for birds and mammals, such as overspray and oral grooming, when conducting environmental risk assessments and take variation in vegetation structure and age-related animal behavior into account, because these factors might affect pesticide exposure. Interactions between pesticide application and vegetation structure may both increase or decrease exposure but can be predicted using a simulation approach.