评价一种用于在人体皮肤产生维生素 D 的紫外线 B 发光二极管 (LED)。
Evaluation of a Ultraviolet B Light Emitting Diode (LED) for Producing Vitamin D in Human Skin.
机构信息
Section of Endocrinology, Diabetes, Nutrition and Weight Management, Department of Medicine, Vitamin D, Skin, and Bone Research Laboratory, Boston University School of Medicine, Boston, MA, U.S.A.
出版信息
Anticancer Res. 2020 Feb;40(2):719-722. doi: 10.21873/anticanres.14002.
AIM
A commercially available light emitting diode (LED) that transmitted narrow band ultraviolet B (UVB) radiation was evaluated for its efficacy and efficiency to produce vitamin D in human skin.
MATERIALS AND METHODS
Human skin samples were obtained from surgical procedures. The LED had peak emission wavelength of 295 nm. Skin samples were exposed to the UVB-LED for varying times and then were analyzed by high-pressure liquid chromatography (HPLC) to determine the vitamin D content.
RESULTS
There was a statistically significant time- and dose-dependent increase in the percent of 7-dehydrocholesterol that was converted to vitamin D in the skin type II samples; 1.3%±0.5, 2.3%±0.6 and 4.5%±1.67 after exposure to 0.75 (11.7 mJ/cm), 1.5 (23.4 mJ/cm) and 3 (46.8 mJ/cm) minimal erythemal doses (MEDs), respectively.
CONCLUSION
The UVB-LED was effective and efficient in generating vitamin D in human skin, in vitro. The amount of vitamin D production increased in a dose-dependent fashion with increased UVB energy. UVB-LEDs can be developed for devices that can efficiently produce vitamin D in human skin.
目的
评估一种市售的窄带发射紫外线 B（UVB）辐射的发光二极管（LED）在人类皮肤中产生维生素 D 的功效和效率。
材料和方法
从手术过程中获得人类皮肤样本。LED 的峰值发射波长为 295nm。将皮肤样本暴露于 UVB-LED 下不同时间，然后通过高压液相色谱（HPLC）进行分析，以确定维生素 D 的含量。
结果
在 II 型皮肤样本中，7-脱氢胆固醇转化为维生素 D 的百分比随时间和剂量呈显著的依赖性增加；分别暴露于 0.75（11.7mJ/cm）、1.5（23.4mJ/cm）和 3（46.8mJ/cm）最小红斑剂量（MED）后，为 1.3%±0.5、2.3%±0.6 和 4.5%±1.67。
结论
UVB-LED 在体外有效且高效地在人类皮肤中产生维生素 D。随着 UVB 能量的增加，维生素 D 的产生量呈剂量依赖性增加。可以开发 UVB-LED 设备，以有效地在人类皮肤中产生维生素 D。
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