瞬时转染生产重组腺相关病毒(rAAVs)。
Production of Recombinant Adeno-Associated Viruses (rAAVs) by Transient Transfection.
出版信息
Cold Spring Harb Protoc. 2020 Feb 3;2020(2):095596. doi: 10.1101/pdb.prot095596.
PMID:32015001
Abstract
The most commonly used method for production of recombinant adeno-associated virus (rAAVs) in research laboratories is by transient triple transfection of 293 cells with AAV and plasmids and an adenovirus helper plasmid. This protocol describes the processes required to prepare the transfected cell suspension for virus purification.
摘要
在研究实验室中，生产重组腺相关病毒（rAAV）最常用的方法是通过瞬时三重转染 293 细胞，使用 AAV 和质粒以及腺病毒辅助质粒。本方案描述了为病毒纯化准备转染细胞悬浮液所需的过程。
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