碘克沙醇梯度离心法纯化重组腺相关病毒（rAAV）。
Purification of Recombinant Adeno-Associated Viruses (rAAVs) by Iodixanol Gradient Centrifugation.
出版信息
Cold Spring Harb Protoc. 2020 Feb 3;2020(2):095612. doi: 10.1101/pdb.prot095612.
PMID:32015002
Abstract
This is a simple method for rapid preparation of recombinant adeno-associated virus (rAAV) stocks, which can be used for in vivo gene delivery. The purity of these vectors is considerably lower than that obtained by either CsCl gradient centrifugation or by combination of iodixanol gradient ultracentrifugation followed by column chromatography.
摘要
这是一种用于快速制备重组腺相关病毒（rAAV）的简单方法，可用于体内基因传递。与 CsCl 梯度离心或碘克沙醇梯度超速离心后结合柱层析法相比，这些载体的纯度要低得多。
Zotero 插件