碘克沙醇梯度离心联合阴离子交换柱层析从柱纯化的重组腺相关病毒（rAAV）制剂中富集完全包装的病毒。
Enrichment of Fully Packaged Virions in Column-Purified Recombinant Adeno-Associated Virus (rAAV) Preparations by Iodixanol Gradient Centrifugation Followed by Anion-Exchange Column Chromatography.
出版信息
Cold Spring Harb Protoc. 2020 Feb 3;2020(2):095638. doi: 10.1101/pdb.prot095638.
PMID:32015003
Abstract
This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.
摘要
本研究提供了一种快速高效的方法来制备高度纯化的重组腺相关病毒（rAAV），该方法基于带负电荷的 rAAV 衣壳与阴离子交换树脂的结合，这种结合依赖于 pH 值。
Zotero 插件