Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, China.
Nat Chem Biol. 2020 May;16(5):489-492. doi: 10.1038/s41589-019-0459-3. Epub 2020 Feb 3.
RNA secondary structure is critical to RNA regulation and function. We report a new N-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.
RNA 二级结构对 RNA 的调控和功能至关重要。我们报告了一种新的 N-酮代乙醛肟试剂，可在活细胞中快速且可逆地标记单链鸟嘌呤碱基。这种基于 N-酮代乙醛肟的化学方法可在温和条件下实现高效的 RNA 标记，并可进行全转录组 RNA 二级结构图谱绘制。
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