• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

鉴定一个潜在的功能性环状RNA-微小RNA-信使RNA调控网络，用于研究膀胱癌的发病机制并提供可能的生物标志物。

Identification of a potentially functional circRNA-miRNA-mRNA regulatory network for investigating pathogenesis and providing possible biomarkers of bladder cancer.

作者信息

Lu Hong-Cheng, Yao Jia-Qi, Yang Xiao, Han Jie, Wang Jing-Zi, Xu Kun, Zhou Rui, Yu Hao, Lv Qiang, Gu Min

机构信息

1Department of Urology, The First Affiliated Hospital of Nanjing Medical University, 300, Guangzhou Road, Nanjing, 210029 Jiangsu China.

2Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, 300, Guangzhou Road, Nanjing, 210029 Jiangsu China.

出版信息

Cancer Cell Int. 2020 Jan 29;20:31. doi: 10.1186/s12935-020-1108-3. eCollection 2020.

DOI:10.1186/s12935-020-1108-3
PMID:32015691
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6990554/
Abstract

BACKGROUND

Circular RNAs (circRNAs) have received considerable attention in human cancer research. However, many circRNAs remain to be detected. In our study, we determined novel circRNAs and investigated their effects on bladder cancer (BCa).

METHODS

Microarray dataset GSE92675 was downloaded from Gene Expression Omnibus (GEO). Then, we combined computational biology with quantitative real-time polymerase chain reaction (qRT-PCR) to select related circRNAs in BCa. The selected circRNA-microRNA (miRNA)-messenger RNA (mRNA) regulatory subnetwork was determined by Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.

RESULTS

The regulatory network constructed from the microarray dataset (GSE92675) contained 49 differentially expressed circRNAs (DECs). GO and KEGG analyses showed that the MAPK and PI3K-AKT signaling pathways were statistically significant. On the basis of qRT-PCR and the degree value calculated by the cytoHubba plugin of Cytoscape, hsa_circ_0011385 was finally confirmed. The subnetwork around hsa_circ_0011385 was constructed. In addition, we created a protein-protein interaction (PPI) network composed of 67 nodes and 274 edges after removing independent nodes. GO and KEGG analyses showed that hubgenes were involved in cell cycle activities. Moreover, they could be regulated by miRNAs and play an eventful role in BCa pathogenesis.

CONCLUSIONS

We proposed a novel circRNA-miRNA-mRNA network related to BCa pathogenesis. This network might be a new molecular biomarker and could be used to develop potential treatment strategies for BCa.

摘要

背景

环状RNA（circRNAs）在人类癌症研究中受到了广泛关注。然而，仍有许多circRNAs有待发现。在我们的研究中，我们鉴定了新型circRNAs，并研究了它们对膀胱癌（BCa）的影响。

方法

从基因表达综合数据库（GEO）下载微阵列数据集GSE92675。然后，我们将计算生物学与定量实时聚合酶链反应（qRT-PCR）相结合，以筛选BCa中相关的circRNAs。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析确定了所选的circRNA-微小RNA（miRNA）-信使RNA（mRNA）调控子网络。

结果

从微阵列数据集（GSE92675）构建的调控网络包含49个差异表达的circRNAs（DECs）。GO和KEGG分析表明，MAPK和PI3K-AKT信号通路具有统计学意义。基于qRT-PCR和Cytoscape的cytoHubba插件计算的度值，最终确认了hsa_circ_0011385。构建了围绕hsa_circ_0011385的子网络。此外，在去除独立节点后，我们创建了一个由67个节点和274条边组成的蛋白质-蛋白质相互作用（PPI）网络。GO和KEGG分析表明，hubgenes参与细胞周期活动。此外，它们可能受miRNAs调控，并在BCa发病机制中发挥重要作用。

结论

我们提出了一个与BCa发病机制相关的新型circRNA-miRNA-mRNA网络。该网络可能是一种新的分子生物标志物，可用于开发BCa的潜在治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/5be168202844/12935_2020_1108_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/0689888f0145/12935_2020_1108_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/0ad92a78d1c3/12935_2020_1108_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/27cbe5fd8a30/12935_2020_1108_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/44bb7e0b85c2/12935_2020_1108_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/9339f046dd9e/12935_2020_1108_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/862c7eae49a6/12935_2020_1108_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/080942c00b6e/12935_2020_1108_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/870757e8d80d/12935_2020_1108_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/685b0c3be92c/12935_2020_1108_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/136c5cb4499a/12935_2020_1108_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/593b6e4479e8/12935_2020_1108_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/14d4615233bc/12935_2020_1108_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/59cda87e035f/12935_2020_1108_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/5be168202844/12935_2020_1108_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/0689888f0145/12935_2020_1108_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/0ad92a78d1c3/12935_2020_1108_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/27cbe5fd8a30/12935_2020_1108_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/44bb7e0b85c2/12935_2020_1108_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/9339f046dd9e/12935_2020_1108_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/862c7eae49a6/12935_2020_1108_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/080942c00b6e/12935_2020_1108_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/870757e8d80d/12935_2020_1108_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/685b0c3be92c/12935_2020_1108_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/136c5cb4499a/12935_2020_1108_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/593b6e4479e8/12935_2020_1108_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/14d4615233bc/12935_2020_1108_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/59cda87e035f/12935_2020_1108_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be18/6990554/5be168202844/12935_2020_1108_Fig14_HTML.jpg

相似文献

1
Identification of a potentially functional circRNA-miRNA-mRNA regulatory network for investigating pathogenesis and providing possible biomarkers of bladder cancer.鉴定一个潜在的功能性环状RNA-微小RNA-信使RNA调控网络，用于研究膀胱癌的发病机制并提供可能的生物标志物。
Cancer Cell Int. 2020 Jan 29;20:31. doi: 10.1186/s12935-020-1108-3. eCollection 2020.
2
Construction of a circRNA-miRNA-mRNA network based on differentially co-expressed circular RNA in gastric cancer tissue and plasma by bioinformatics analysis.基于生物信息学分析构建胃癌组织和血浆差异共表达 circRNA 的 circRNA-miRNA-mRNA 网络。
World J Surg Oncol. 2022 Feb 14;20(1):34. doi: 10.1186/s12957-022-02503-7.
3
Identification of Potentially Functional CircRNA-miRNA-mRNA Regulatory Network in Hepatocellular Carcinoma by Integrated Microarray Analysis.通过综合微阵列分析鉴定肝细胞癌中潜在的功能性环状RNA-微小RNA-信使RNA调控网络
Med Sci Monit Basic Res. 2018 Apr 30;24:70-78. doi: 10.12659/MSMBR.909737.
4
Identification of the circRNA-miRNA-mRNA regulatory network and its prognostic effect in colorectal cancer.环状RNA-微小RNA-信使核糖核酸调控网络的鉴定及其在结直肠癌中的预后作用
World J Clin Cases. 2021 Jun 26;9(18):4520-4541. doi: 10.12998/wjcc.v9.i18.4520.
5
Construction of Circular RNA-MicroRNA-Messenger RNA Regulatory Network of Recurrent Implantation Failure to Explore Its Potential Pathogenesis.复发性植入失败的环状RNA-微小RNA-信使RNA调控网络构建以探索其潜在发病机制
Front Genet. 2021 Feb 16;11:627459. doi: 10.3389/fgene.2020.627459. eCollection 2020.
6
Identification of Serum Exosome-Derived circRNA-miRNA-TF-mRNA Regulatory Network in Postmenopausal Osteoporosis Using Bioinformatics Analysis and Validation in Peripheral Blood-Derived Mononuclear Cells.基于生物信息学分析和外周血单核细胞验证鉴定绝经后骨质疏松症血清外泌体来源 circRNA-miRNA-TF-mRNA 调控网络
Front Endocrinol (Lausanne). 2022 Jun 9;13:899503. doi: 10.3389/fendo.2022.899503. eCollection 2022.
7
Identification of a potentially functional circRNA-miRNA-mRNA ceRNA regulatory network in bladder cancer by analysis of microarray data.通过微阵列数据分析鉴定膀胱癌中一个潜在具有功能的环状RNA-微小RNA-信使核糖核酸竞争性内源RNA调控网络
Transl Androl Urol. 2021 Jan;10(1):24-36. doi: 10.21037/tau-20-660.
8
Integrated analyses reveal hsa_circ_0028883 as a diagnostic biomarker in active tuberculosis.综合分析显示 hsa_circ_0028883 可作为活动性结核病的诊断生物标志物。
Infect Genet Evol. 2020 Sep;83:104323. doi: 10.1016/j.meegid.2020.104323. Epub 2020 Apr 16.
9
Bioinformatics Analysis Predicts hsa_circ_0026337/miR-197-3p as a Potential Oncogenic ceRNA Network for Non-Small Cell Lung Cancers.生物信息学分析预测 hsa_circ_0026337/miR-197-3p 作为非小细胞肺癌的潜在致癌 ceRNA 网络。
Anticancer Agents Med Chem. 2022;22(5):874-886. doi: 10.2174/1871520621666210712090721.
10
Construction of the circRNA-miRNA-mRNA Regulatory Network of an Abdominal Aortic Aneurysm to Explore Its Potential Pathogenesis.构建腹主动脉瘤的 circRNA-miRNA-mRNA 调控网络，探索其潜在的发病机制。
Dis Markers. 2021 Nov 5;2021:9916881. doi: 10.1155/2021/9916881. eCollection 2021.

引用本文的文献

1
Construction of circRNA-miRNA-mRNA ceRNA regulatory network and screening of diagnostic targets for tuberculosis.环状 RNA-miRNA-mRNA ceRNA 调控网络的构建及结核诊断靶标的筛选。
Ann Med. 2024 Dec;56(1):2416604. doi: 10.1080/07853890.2024.2416604. Epub 2024 Oct 22.
2
CircMCTP2 enhances the progression of bladder cancer by regulating the miR-99a-5p/FZD8 axis.环状 RNA MCTP2 通过调控 miR-99a-5p/FZD8 轴促进膀胱癌的进展。
J Egypt Natl Canc Inst. 2024 Mar 18;36(1):8. doi: 10.1186/s43046-024-00206-6.
3
Downregulated M6A modification and expression of circRNA_103239 promoted the progression of glioma by regulating the miR-182-5p/MTSS1 signalling pathway.

本文引用的文献

1
RAB14 activates MAPK signaling to promote bladder tumorigenesis.RAB14 通过激活 MAPK 信号通路促进膀胱癌发生。
Carcinogenesis. 2019 Nov 25;40(11):1341-1351. doi: 10.1093/carcin/bgz039.
2
Circular RNA cTFRC acts as the sponge of MicroRNA-107 to promote bladder carcinoma progression.环状 RNA cTFRC 作为 MicroRNA-107 的海绵体促进膀胱癌进展。
Mol Cancer. 2019 Feb 19;18(1):27. doi: 10.1186/s12943-019-0951-0.
3
Hypoxia-elevated circELP3 contributes to bladder cancer progression and cisplatin resistance.低氧上调的 circELP3 促进膀胱癌进展和顺铂耐药性。
下调的m6A修饰和circRNA_103239的表达通过调节miR-182-5p/MTSS1信号通路促进了胶质瘤的进展。
J Cancer. 2023 Oct 16;14(18):3508-3520. doi: 10.7150/jca.85320. eCollection 2023.
4
Comprehensive Analysis of circRNA-Mediated ceRNA Regulatory Networks in relation to Recurrent Implantation Failure.与反复种植失败相关的环状RNA介导的ceRNA调控网络的综合分析
Evid Based Complement Alternat Med. 2022 Aug 23;2022:8314838. doi: 10.1155/2022/8314838. eCollection 2022.
5
An Integrated Bioinformatics Analysis towards the Identification of Diagnostic, Prognostic, and Predictive Key Biomarkers for Urinary Bladder Cancer.用于鉴定膀胱癌诊断、预后和预测关键生物标志物的综合生物信息学分析
Cancers (Basel). 2022 Jul 10;14(14):3358. doi: 10.3390/cancers14143358.
6
Circ_0011385 knockdown inhibits cell proliferation, migration and invasion, whereas promotes cell apoptosis by regulating miR-330-3p/MYO6 axis in colorectal cancer.Circ_0011385 敲低通过调控 miR-330-3p/MYO6 轴抑制结直肠癌细胞增殖、迁移和侵袭，促进细胞凋亡。
Biomed J. 2023 Feb;46(1):110-121. doi: 10.1016/j.bj.2022.01.007. Epub 2022 Jan 26.
7
Downregulation of hsa_circRNA_0001400 Helps to Promote Cell Apoptosis Through Disruption of the circRNA_0001400-miR-326 Sponge in Cervical Cancer Cells.hsa_circRNA_0001400的下调通过破坏环状RNA_0001400- miR - 326海绵体促进宫颈癌细胞凋亡。
Front Genet. 2021 Dec 17;12:779195. doi: 10.3389/fgene.2021.779195. eCollection 2021.
8
Circular RNA regulates the onset and progression of cancer through the mitogen-activated protein kinase signaling pathway.环状RNA通过丝裂原活化蛋白激酶信号通路调控癌症的发生和发展。
Oncol Lett. 2021 Dec;22(6):817. doi: 10.3892/ol.2021.13078. Epub 2021 Oct 5.
9
A network pharmacology approach to determine the underlying mechanisms of action of Yishen Tongluo formula for the treatment of oligoasthenozoospermia.网络药理学方法探讨益肾通络方治疗少弱精子症的作用机制。
PLoS One. 2021 Jun 21;16(6):e0252906. doi: 10.1371/journal.pone.0252906. eCollection 2021.
10
Transcriptome analyses of urine RNA reveal tumor markers for human bladder cancer: validated amplicons for RT-qPCR-based detection.尿液RNA的转录组分析揭示了人类膀胱癌的肿瘤标志物：基于RT-qPCR检测的验证扩增子
Oncotarget. 2021 May 11;12(10):1011-1023. doi: 10.18632/oncotarget.27954.
Int J Biol Sci. 2019 Jan 1;15(2):441-452. doi: 10.7150/ijbs.26826. eCollection 2019.
4
Semaphorin 4D promotes the proliferation and metastasis of bladder cancer by activating the PI3K/AKT pathway.信号素4D通过激活PI3K/AKT信号通路促进膀胱癌的增殖和转移。
Tumori. 2019 Jun;105(3):231-242. doi: 10.1177/0300891618811280. Epub 2019 Jan 24.
5
Circular RNA circMTO1 suppresses bladder cancer metastasis by sponging miR-221 and inhibiting epithelial-to-mesenchymal transition.环状 RNA circMTO1 通过海绵吸附 miR-221 并抑制上皮-间充质转化来抑制膀胱癌转移。
Biochem Biophys Res Commun. 2019 Jan 22;508(4):991-996. doi: 10.1016/j.bbrc.2018.12.046. Epub 2018 Dec 11.
6
Circular RNA BCRC-3 suppresses bladder cancer proliferation through miR-182-5p/p27 axis.环状 RNA BCRC-3 通过 miR-182-5p/p27 轴抑制膀胱癌增殖。
Mol Cancer. 2018 Oct 3;17(1):144. doi: 10.1186/s12943-018-0892-z.
7
Circular RNA circ-ITCH inhibits bladder cancer progression by sponging miR-17/miR-224 and regulating p21, PTEN expression.环状 RNA circ-ITCH 通过海绵吸附 miR-17/miR-224 并调节 p21、PTEN 表达抑制膀胱癌进展。
Mol Cancer. 2018 Jan 31;17(1):19. doi: 10.1186/s12943-018-0771-7.
8
CSCD: a database for cancer-specific circular RNAs.CSCD：一个用于癌症特异性环状 RNA 的数据库。
Nucleic Acids Res. 2018 Jan 4;46(D1):D925-D929. doi: 10.1093/nar/gkx863.
9
Circular RNA MYLK as a competing endogenous RNA promotes bladder cancer progression through modulating VEGFA/VEGFR2 signaling pathway.环状RNA MYLK作为一种竞争性内源性RNA，通过调节VEGFA/VEGFR2信号通路促进膀胱癌进展。
Cancer Lett. 2017 Sep 10;403:305-317. doi: 10.1016/j.canlet.2017.06.027. Epub 2017 Jul 4.
10
Bladder Cancer Incidence and Mortality: A Global Overview and Recent Trends.膀胱癌发病率和死亡率：全球概述及最新趋势。
Eur Urol. 2017 Jan;71(1):96-108. doi: 10.1016/j.eururo.2016.06.010. Epub 2016 Jun 28.