Wang Hanbing, Lv Wei, Zhu Yilin
Department of Gynecology and Obstetrics, Wuhan No. 1 Hospital, Wuhan, China.
Evid Based Complement Alternat Med. 2022 Aug 23;2022:8314838. doi: 10.1155/2022/8314838. eCollection 2022.
Recurrent implantation failure (RIF) is attributed to endometrial receptivity dysfunction with many unanswered questions. Up to now, there is no explanation for RIF, and therapeutic strategies are usually limited to supportive care. In this study, we differentially analyzed the raw data deposited in three eligible microarray datasets, GSE111974, GSE121219, and GSE147442 to screen DE-mRNAs, DE-miRNAs, and DE-circRNAs, respectively. The value of log2-fold change |log2FC| ≥ 1 and the adjusted value < 0.05 were considered differentially expressed between RIF and fertile control. We found 350 DE-mRNAs, 43 DE-miRNAs, and 1968 DE-circRNAs between RIF and fertile control. The PPI network identified 6 hub genes with degree ≥10, KDR, AGT, POSTN, TOP2A, RRM2, and PTGS2, in RIF. KDR, AGT, POSTN, TOP2A, and RRM2 were downregulated in endometrial tissue samples of RIF compared with those of fertile control, while PTGS2 was upregulated in endometrial tissue samples of RIF compared with those of fertile control. According to the ceRNA hypothesis, 15 groups of ceRNA network based on 10 circRNAs, hsa_circ_001572, hsa_circ_001884, hsa_circ_001375, hsa_circ_001449, hsa_circ_000029, hsa_circ_001168, hsa_circ_000210, hsa_circ_001484, hsa_circ_001698, and hsa_circ_000089 were constructed in RIF. In conclusion, the present study examined the possible role of circRNAs and their related ceRNA network involved in the pathogenesis of RIF.
反复种植失败（RIF）归因于子宫内膜容受性障碍，仍有许多问题未得到解答。到目前为止，RIF尚无明确解释，治疗策略通常仅限于支持治疗。在本研究中，我们对存入三个符合条件的微阵列数据集GSE111974、GSE121219和GSE147442中的原始数据进行差异分析，分别筛选差异表达的信使核糖核酸（DE-mRNAs）、微小核糖核酸（DE-miRNAs）和环状核糖核酸（DE-circRNAs）。RIF组与生育力正常对照组之间，log2倍数变化值|log2FC|≥1且校正值<0.05被视为差异表达。我们发现RIF组与生育力正常对照组之间有350个DE-mRNAs、43个DE-miRNAs和1968个DE-circRNAs。蛋白质-蛋白质相互作用（PPI）网络确定了RIF组中6个度≥10的枢纽基因，即激酶插入域受体（KDR）、血管紧张素原（AGT）、骨膜蛋白（POSTN）、拓扑异构酶IIα（TOP2A）、核糖核苷酸还原酶M2亚基（RRM2）和前列腺素内过氧化物合酶2（PTGS2）。与生育力正常对照组相比，RIF组子宫内膜组织样本中KDR、AGT、POSTN、TOP2A和RRM2表达下调，而PTGS2表达上调。根据竞争性内源性RNA（ceRNA）假说，在RIF组中基于10个环状核糖核酸，即hsa_circ_001572、hsa_circ_001884、hsa_circ_001375、hsa_circ_001449、hsa_circ_000029、hsa_circ_001168、hsa_circ_000210、hsa_circ_001484、hsa_circ_001698和hsa_circ_000089构建了15组ceRNA网络。总之，本研究探讨了环状核糖核酸及其相关ceRNA网络在RIF发病机制中的可能作用。
