利用高通量测序鉴定[具体物种或组织等，原文未明确]中的干旱响应微小RNA。
Identification of drought-responsive miRNAs in using high-throughput sequencing.
作者信息
Fan Gang, Liu Yue, Du Huan, Kuang Tingting, Zhang Yi
机构信息
1College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137 China.
2Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, 610051 China.
出版信息
3 Biotech. 2020 Feb;10(2):53. doi: 10.1007/s13205-019-2045-5. Epub 2020 Jan 21.
MicroRNAs (miRNAs) play an important role in abiotic stress response in plants. However, the total miRNA profiles (miRNome) and drought-responsive miRNAs in have not been identified. In this study, we present the first report on the miRNome profiles of by high-throughput sequencing technology. 116 known and 4 predicted novel miRNAs were all identified in six samples. Moreover, to reveal the drought-responsive miRNAs in , we compared the miRNA profiles of grown under water sufficiency and drought stress. The results showed that 39 known miRNAs were up-regulated, while 34 miRNAs were downregulated under drought stress. Moreover, the expression of two novel miRNAs (novel_mir_24 and novel_mir_87) showed notable changes in response to drought stress. The target genes of these differentially expressed miRNAs were mainly enriched in cellular process, metabolic process, cell part, and response to stimulus. The identified drought-responsive miRNAs might be used for improving drought tolerance in and other plateau plants.
微小RNA（miRNA）在植物非生物胁迫响应中发挥着重要作用。然而，尚未鉴定出[植物名称]的总miRNA谱（miRNome）和干旱响应性miRNA。在本研究中，我们通过高通量测序技术首次报道了[植物名称]的miRNome谱。在六个[植物名称]样本中总共鉴定出116个已知miRNA和4个预测的新miRNA。此外，为了揭示[植物名称]中干旱响应性miRNA，我们比较了在水分充足和干旱胁迫下生长的[植物名称]的miRNA谱。结果表明，在干旱胁迫下，39个已知miRNA上调，而34个miRNA下调。此外，两个新miRNA（novel_mir_24和novel_mir_87）的表达在干旱胁迫下表现出显著变化。这些差异表达miRNA的靶基因主要富集在细胞过程、代谢过程、细胞部分和对刺激的反应中。鉴定出的干旱响应性miRNA可用于提高[植物名称]和其他高原植物的耐旱性。
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