Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Henan, China.
Department of Orthopaedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.
Biomed Res Int. 2020 Jan 18;2020:6247489. doi: 10.1155/2020/6247489. eCollection 2020.
The Corneal wound healing results in the formation of opaque corneal scar. In fact, millions of people around the world suffer from corneal scars, leading to loss of vision. This study aimed to identify the key changes of gene expression in the formation of opaque corneal scar and provided potential biomarker candidates for clinical treatment and drug target discovery. We downloaded Gene expression dataset GSE6676 from NCBI-GEO, and analyzed the Differentially Expressed Genes (DEGs), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analyses, and protein-protein interaction (PPI) network. A total of 1377 differentially expressed genes were identified and the result of Functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) identification and protein-protein interaction (PPI) networks were performed. In total, 7 hub genes IL6 (interleukin-6), MMP9 (matrix metallopeptidase 9), CXCL10 (C-X-C motif chemokine ligand 10), MAPK8 (mitogen-activated protein kinase 8), TLR4 (toll-like receptor 4), HGF (hepatocyte growth factor), EDN1 (endothelin 1) were selected. In conclusion, the DEGS, Hub genes and signal pathways identified in this study can help us understand the molecular mechanism of corneal scar formation and provide candidate targets for the diagnosis and treatment of corneal scar.
角膜伤口愈合会导致混浊性角膜瘢痕的形成。事实上，全世界有数百万的人患有角膜瘢痕，导致视力丧失。本研究旨在确定混浊性角膜瘢痕形成过程中基因表达的关键变化，并为临床治疗和药物靶点发现提供潜在的生物标志物候选物。我们从 NCBI-GEO 下载了基因表达数据集 GSE6676，并对差异表达基因（DEGs）、基因本体论（GO）、京都基因与基因组百科全书（KEGG）富集途径分析以及蛋白质-蛋白质相互作用（PPI）网络进行了分析。共鉴定出 1377 个差异表达基因，并对其进行了功能富集分析、京都基因与基因组百科全书（KEGG）鉴定和蛋白质-蛋白质相互作用（PPI）网络分析。总共选择了 7 个关键基因：白细胞介素 6（IL6）、基质金属蛋白酶 9（MMP9）、C-X-C 基序趋化因子配体 10（CXCL10）、丝裂原活化蛋白激酶 8（MAPK8）、Toll 样受体 4（TLR4）、肝细胞生长因子（HGF）和内皮素 1（EDN1）。总之，本研究中鉴定的差异表达基因、关键基因和信号通路可以帮助我们了解角膜瘢痕形成的分子机制，并为角膜瘢痕的诊断和治疗提供候选靶点。
