Department of Otolaryngology Head and Neck Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101199, P.R. China.
Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100043, P.R. China.
Mol Med Rep. 2020 Mar;21(3):1640-1646. doi: 10.3892/mmr.2020.10960. Epub 2020 Jan 24.
Following peripheral nerve injury, factors in the local microenvironment can induce the differentiation of bone marrow‑derived mesenchymal stem cells (BMSCs) into Schwann cells; however, the specific factors that participate in this process remain unclear. The present study aimed to investigate the role of Schwann cell‑derived exosomes in the differentiation of BMSCs into Schwann cells. Exosomes were extracted from Schwann cells or fibroblasts and co‑cultured with BMSCs. The morphology, as well as gene and protein expressions of the BMSCs were measured to determine the effect of exosomes on cell differentiation. The levels of Schwann cell‑specific markers in BMSCs were significantly increased by Schwann cell‑derived exosomes compared with untreated BMSCs; however, fibroblast‑derived exosomes did not demonstrate the same effects. In conclusion, Schwann cell‑derived exosomes may be involved in the differentiation of BMSCs into Schwann cells, which may provide a novel target for promoting nerve regeneration following injury.
在周围神经损伤后，局部微环境中的因素可诱导骨髓间充质干细胞（BMSCs）分化为施万细胞；然而，参与这一过程的确切因素仍不清楚。本研究旨在探讨施万细胞衍生的外泌体在 BMSCs 分化为施万细胞中的作用。从施万细胞或成纤维细胞中提取外泌体，并与 BMSCs 共培养。测量 BMSCs 的形态以及基因和蛋白表达，以确定外泌体对细胞分化的影响。与未处理的 BMSCs 相比，施万细胞衍生的外泌体可显著增加 BMSCs 中施万细胞特异性标志物的水平；然而，成纤维细胞衍生的外泌体则没有相同的作用。综上所述，施万细胞衍生的外泌体可能参与 BMSCs 分化为施万细胞，这可能为促进损伤后神经再生提供新的靶点。
