Pharmacology Division, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh, 160014, India.
Pharmacology Research Laboratory, UGC Centre of Advanced Studies, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh, 160014, India.
Pharmacol Rep. 2020 Feb;72(1):104-114. doi: 10.1007/s43440-019-00022-z. Epub 2019 Dec 19.
Astaxanthin (ATX), a natural xanthophyll carotenoid, has shown to exert significant protective effects against various diseases via its antioxidant and anti-inflammatory properties. However, its potential role in arthritis is still not reported. Therefore, the aim of the present study was to investigate the potential anti-arthritic properties of ATX against complete Freund's adjuvant (CFA)-induced arthritis rats.
Adjuvant arthritis was induced by single intraplantar injection of complete Freund's adjuvant (CFA) in the left hind paw of adult female Wistar rats. ATX (25, 50 and 100 mg/kg) and indomethacin (5 mg/kg) were given orally from days 14 to 28. The anti-arthritic activity was evaluated through various nociceptive behavioral tests (mechanical allodynia, mechanical hyperalgesia, cold allodynia, and thermal hyperalgesia), paw edema assessment, and arthritis scores. Serum tumor necrosis factor-α (TNF-α), C-reactive protein (CRP) and cyclic citrullinated peptide (CCP) antibody levels were assessed. Moreover, malondialdehyde (MDA), nitrite, glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels were also evaluated.
Oral administration of ATX (50 and 100 mg/kg) exhibited significant anti-arthritic activity via enhancing the nociceptive threshold, reducing paw edema and improving arthritis scores. Moreover, ATX treatment also markedly suppressed inflammatory and oxidative mediators in adjuvant-administered rats.
Our findings suggest that ATX possesses potential anti-arthritic activity, which could be attributed to its anti-inflammatory and antioxidant properties.
虾青素（ATX）是一种天然类胡萝卜素叶黄素，具有抗氧化和抗炎特性，已被证明对各种疾病具有显著的保护作用。然而，其在关节炎中的潜在作用尚未报道。因此，本研究旨在探讨 ATX 对完全弗氏佐剂（CFA）诱导的关节炎大鼠的潜在抗关节炎作用。
通过在成年雌性 Wistar 大鼠左后爪内单次注射完全弗氏佐剂（CFA）诱导关节炎。ATX（25、50 和 100mg/kg）和吲哚美辛（5mg/kg）从第 14 天到第 28 天口服给药。通过各种伤害感受性行为测试（机械性痛觉过敏、机械性痛觉超敏、冷性痛觉过敏和热性痛觉过敏）、爪肿胀评估和关节炎评分评估抗关节炎活性。评估血清肿瘤坏死因子-α（TNF-α）、C 反应蛋白（CRP）和环瓜氨酸肽（CCP）抗体水平。此外，还评估了丙二醛（MDA）、亚硝酸盐、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平。
ATX（50 和 100mg/kg）口服给药通过提高痛觉阈值、减少爪肿胀和改善关节炎评分表现出显著的抗关节炎活性。此外，ATX 治疗还显著抑制了佐剂给药大鼠的炎症和氧化介质。
我们的研究结果表明，ATX 具有潜在的抗关节炎活性，这可能归因于其抗炎和抗氧化特性。
