Pena Eduardo, El Alam Samia, Gonzalez Constanza, Cortés Isaac, Aguilera Diego, Flores Karen, Arriaza Karem
High Altitude Medicine Research Center (CEIMA), Arturo Prat University, Iquique 1100000, Chile.
Science Faculty, Arturo Prat University, Iquique 1100000, Chile.
Antioxidants (Basel). 2024 Oct 18;13(10):1269. doi: 10.3390/antiox13101269.
In Chile, individuals are commonly exposed to high altitude due to the work shift system, involving days of exposure to high altitude followed by days at sea level over the long term, which can result in chronic intermittent hypobaric hypoxia (CIHH). CIHH can cause high-altitude pulmonary hypertension (HAPH), the principal manifestation of which is right ventricular hypertrophy (RVH), in some cases leading to heart failure and eventually death. Studies have shown the contribution of oxidative stress and inflammation to RVH development. Recently, it was determined that the pigment astaxanthin has high antioxidant capacity and strong anti-inflammatory and cardioprotective effects. Therefore, the aim of this study was to determine the effects of astaxanthin on RVH development in rats subjected to CIHH.
Thirty two male Wistar rats were randomly assigned to the following groups (n = 8 per group): the normoxia with vehicle (NX), normoxia with astaxanthin (NX + AS), chronic intermittent hypobaric hypoxia with vehicle (CIHH), and chronic intermittent hypobaric hypoxia with astaxanthin (CIHH + AS) groups. CIHH was simulated by 2 days in a hypobaric chamber followed by 2 days at sea level for 29 days.
Exposure to CIHH induced RVH and increased lipid peroxidation (MDA), Nox2 expression, and SOD activity, however, it decreased pro-IL-1β expression. Astaxanthin restored oxidative stress markers (Nox2 and MDA), increased GPx activity, and decreased RVH compared to CIHH.
Astaxanthin alleviates RVH and reduces Nox2 and MDA levels while increasing GPx activity in rats subjected to CIHH. These findings provide new insights of astaxanthin as a new nutraceutical against high-altitude effects.
在智利，由于轮班工作制度，人们经常暴露于高海拔环境，长期经历数天的高海拔暴露后接着数天的海平面环境，这可能导致慢性间歇性低压缺氧（CIHH）。CIHH可引发高原性肺动脉高压（HAPH），其主要表现为右心室肥厚（RVH），在某些情况下会导致心力衰竭并最终死亡。研究表明氧化应激和炎症对RVH的发展有影响。最近，已确定色素虾青素具有高抗氧化能力以及强大的抗炎和心脏保护作用。因此，本研究的目的是确定虾青素对CIHH大鼠RVH发展的影响。
32只雄性Wistar大鼠被随机分为以下几组（每组n = 8）：常氧加赋形剂组（NX）、常氧加虾青素组（NX + AS）、慢性间歇性低压缺氧加赋形剂组（CIHH）和慢性间歇性低压缺氧加虾青素组（CIHH + AS）。通过在低压舱中暴露2天，随后在海平面暴露2天，持续29天来模拟CIHH。
暴露于CIHH会诱导RVH，并增加脂质过氧化（MDA）、Nox2表达和SOD活性，然而，它会降低前白细胞介素 - 1β表达。与CIHH相比，虾青素恢复了氧化应激标志物（Nox2和MDA），增加了GPx活性，并减轻了RVH。
虾青素可减轻CIHH大鼠的RVH，降低Nox2和MDA水平，同时增加GPx活性。这些发现为虾青素作为一种对抗高海拔影响的新型营养保健品提供了新的见解。
