离体培养茅膏菜中 3-O-乙酰车叶草酸对癌细胞存活和迁移的影响。
Effect of 3-O-acetylaleuritolic acid from in vitro-cultured Drosera spatulata on cancer cells survival and migration.
机构信息
Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.
Department of Pharmaceutical Botany, Poznan University of Medical Sciences, Marii Magdaleny 14, 61-861, Poznan, Poland.
出版信息
Pharmacol Rep. 2020 Feb;72(1):166-178. doi: 10.1007/s43440-019-00008-x. Epub 2020 Jan 10.
BACKGROUND
Drosera spatulata is a source of many compounds such as naphthoquinones, phenolic acids, flavonoids, anthocyanins, and naphthalene derivatives. Unfortunately, the information regarding the biological activity and chemical profile of those compounds is still incomplete. Herein, we investigated the biological activity of 3-O-acetylaleuritolic acid (3-O-AAA) in cancer cell lines.
METHODS
The cell viability of HeLa, HT-29, MCF7, and MCF12A cells was assessed using MTT assay. Proliferation potential was assessed using the clonogenic assay and flow cytometry. Migration modulation was tested using a scratch assay. Protein expression was analyzed by immunoblotting.
RESULTS
3-O-AAA significantly inhibited the growth of all tested tumor cells. The results of the colony formation assay suggested cytostatic properties of the studied compound. The scratch assay showed that 3-O-AAA was an efficient migration inhibitor in a dose-dependent manner. Moreover, it caused modulation of mTOR, beclin1, and Atg5 proteins suggesting a possible role of the compound in autophagy induction.
CONCLUSION
Collectively, these results demonstrated that 3-O-AAA inhibited the proliferation and migration of cancer cell lines as well as contributed to autophagy induction showing some anticancer properties.
背景
茅膏菜是许多化合物的来源，如萘醌、酚酸、类黄酮、花青素和萘衍生物。不幸的是，关于这些化合物的生物活性和化学特征的信息仍然不完整。在此，我们研究了 3-O-乙酰 Aleuritolic 酸（3-O-AAA）在癌细胞系中的生物活性。
方法
使用 MTT 测定法评估 HeLa、HT-29、MCF7 和 MCF12A 细胞的细胞活力。使用集落形成测定法和流式细胞术评估增殖潜力。通过划痕试验测试迁移调节。通过免疫印迹分析蛋白质表达。
结果
3-O-AAA 显著抑制了所有测试肿瘤细胞的生长。集落形成试验的结果表明研究化合物具有细胞抑制特性。划痕试验表明，3-O-AAA 以剂量依赖性方式有效抑制迁移。此外，它还导致 mTOR、beclin1 和 Atg5 蛋白的调节，表明该化合物可能在自噬诱导中发挥作用。
结论
总之，这些结果表明 3-O-AAA 抑制了癌细胞系的增殖和迁移，并促进了自噬诱导，显示出一些抗癌特性。
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