Shenzhen Mindray Biomedical Electronics Co., Ltd., Shenzhen, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):784-792. doi: 10.26355/eurrev_202001_20061.
To examine the expression level of HOTAIR in pancreatic β cells. Moreover, regulatory effects of HOTAIR on insulin secretion, proliferation, cell cycle, and apoptosis in β cells are determined.
HOTAIR levels in mouse primary pancreatic cells and MIN6 cell line were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Its level was significantly observed in MIN6 cells treated with different doses of glucose. After the knockdown of HOTAIR, insulin secretion, cell cycle distribution, proliferation, and apoptosis in pancreatic β cells were assessed.
HOTAIR was abundantly expressed in pancreatic islets. HOTAIR level in pancreatic tissues of db/db mice was downregulated and influenced by glucose level. Knockdown of HOTAIR attenuated insulin secretion and synthesis capacities in both MIN6 cells and primary pancreatic cells, which may be related by the downregulation of MafA, Pdx1, and NeuroD. Moreover, the silence of HOTAIR suppressed proliferation, arrested cell cycle, and stimulated apoptosis in pancreatic β cells.
HOTAIR is highly expressed in pancreatic tissues. The silence of HOTAIR inhibits insulin secretion by downregulating insulin transcription-related genes. In addition, the silence of HOTAIR suppresses proliferation, arrests cell cycle progression, and induces apoptosis in pancreatic β cells.
检测 HOTAIR 在胰腺β细胞中的表达水平。此外，还确定了 HOTAIR 对β细胞胰岛素分泌、增殖、细胞周期和凋亡的调节作用。
通过实时定量聚合酶链反应（qRT-PCR）测定小鼠原代胰腺细胞和 MIN6 细胞系中的 HOTAIR 水平。观察 MIN6 细胞在不同剂量葡萄糖处理后的 HOTAIR 水平。敲低 HOTAIR 后，评估胰腺β细胞的胰岛素分泌、细胞周期分布、增殖和凋亡。
HOTAIR 在胰岛中大量表达。db/db 小鼠胰腺组织中的 HOTAIR 水平下调，受葡萄糖水平影响。敲低 HOTAIR 可减弱 MIN6 细胞和原代胰腺细胞的胰岛素分泌和合成能力，这可能与 MafA、Pdx1 和 NeuroD 的下调有关。此外，HOTAIR 的沉默抑制了胰腺β细胞的增殖，阻滞了细胞周期，并刺激了细胞凋亡。
HOTAIR 在胰腺组织中高度表达。沉默 HOTAIR 通过下调胰岛素转录相关基因抑制胰岛素分泌。此外，沉默 HOTAIR 抑制胰腺β细胞的增殖、阻滞细胞周期进程并诱导其凋亡。
