Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, 210029, China.
Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
Int J Biochem Cell Biol. 2018 Nov;104:171-179. doi: 10.1016/j.biocel.2018.09.004. Epub 2018 Sep 7.
LncRNAs have been reported to maintain islet function and are associated with the development of diabetes. Here, we investigated Lincpint biological functions in mouse pancreatic β cells both in vivo and in vitro. We observed that Lincpint was highly expressed in BALB/c mouse islets and downregulated in db/db mouse islets by using qRT-PCR. Lincpint could be regulated by different concentrations of glucose in MIN6 cells. MTT and flow cytometry showed that silencing Lincpint expression in vitro increased cell apoptosis. In a GSIS assay, we detected that inhibition of the expression of Lincpint suppressed insulin synthesis and secretion. In male BALB/c mouse, blood glucose and serum insulin were decreased after knockdown of Lincpint expression as detected by IPGTT and ELISA. Immunohistochemistry indicated that positive islet area and expression of Pdx1 and Glut2 was reduced after Lincpint silencing in male BALB/c mouse. These findings suggest that Lincpint may play an important role in maintaining islet β cell function and is worthy of further investigation due to its potential in diabetes treatment.
LncRNAs 已被报道能维持胰岛功能，并与糖尿病的发展有关。在这里，我们研究了 Lincpint 在体内和体外的小鼠胰腺 β 细胞中的生物学功能。我们通过 qRT-PCR 观察到，Lincpint 在 BALB/c 小鼠胰岛中高表达，而在 db/db 小鼠胰岛中低表达。Lincpint 可以在 MIN6 细胞中被不同浓度的葡萄糖调节。MTT 和流式细胞术显示，体外沉默 Lincpint 的表达会增加细胞凋亡。在 GSIS 测定中，我们检测到抑制 Lincpint 的表达会抑制胰岛素的合成和分泌。在雄性 BALB/c 小鼠中，通过 IPGTT 和 ELISA 检测到敲低 Lincpint 表达后，血糖和血清胰岛素降低。免疫组织化学显示，在雄性 BALB/c 小鼠中沉默 Lincpint 后，胰岛的阳性面积和 Pdx1、Glut2 的表达减少。这些发现表明，Lincpint 可能在维持胰岛 β 细胞功能方面发挥重要作用，由于其在糖尿病治疗中的潜力，值得进一步研究。
