Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):878-884. doi: 10.26355/eurrev_202001_20072.
To explore the mechanism underlying micro ribonucleic acid (miR)-21 in the invasion of rat aortic aneurysm cells in vitro by regulating matrix metalloproteinase (MMP)-2 and MMP-9.
Rats were randomly divided into three groups: control group, model group, and miR-21 group. Real Time fluorescence quantitative Polymerase Chain Reaction (qRT-PCR) was adopted to detect the levels of miR-21 in each group of cells, transwell assay was performed to measure the effect of miR-21 on the invasion of aortic aneurysm cells. Western blotting was used to examine the expression of PTEN, which is the predicted target of miR-21 in aortic aneurysm cells, as well as the expressions of invasion-related proteases, MMP-2 and MMP-9.
The expression level of miR-21 in thoracic aortic aneurysm cells in model group was significantly higher than that in normal group (p<0.05), and that in miR-21 group was remarkably higher than that in model group (p<0.05). MiR-21 group had evidently more aortic aneurysm cells and stronger cell invasion ability than normal group and model group (p<0.05). In addition, the expression level of PTEN in model group was significantly higher than that in normal group (p<0.05), while that in miR-21 group notably declined compared to model group, (p<0.05). Compared with normal group and model group, the expressions of MMP-2 and MMP-9 were markedly increased in miR-21 group (p<0.05).
In aortic aneurysm cells of rats, miR-21 could suppress the expression of PTEN and activate MMP-2 and MMP-9 signals to promote the proliferation and migration of aortic aneurysm cells.
通过调控基质金属蛋白酶（MMP）-2 和 MMP-9，探讨微小核糖核酸（miR）-21 调控大鼠主动脉瘤细胞体外侵袭的作用机制。
大鼠随机分为三组：对照组、模型组和 miR-21 组。采用实时荧光定量聚合酶链反应（qRT-PCR）检测各组细胞中 miR-21 的水平，Transwell 小室法检测 miR-21 对主动脉瘤细胞侵袭的影响，Western blot 检测 miR-21 靶基因 PTEN 及与侵袭相关的蛋白酶 MMP-2、MMP-9 在主动脉瘤细胞中的表达。
模型组大鼠胸主动脉瘤细胞中 miR-21 的表达水平明显高于正常组（p<0.05），miR-21 组明显高于模型组（p<0.05）。miR-21 组大鼠胸主动脉瘤细胞数量明显多于正常组和模型组，细胞侵袭能力明显增强（p<0.05）。模型组大鼠主动脉瘤细胞中 PTEN 的表达水平明显高于正常组（p<0.05），miR-21 组明显低于模型组（p<0.05）。与正常组和模型组相比，miR-21 组 MMP-2 和 MMP-9 的表达明显增加（p<0.05）。
在大鼠主动脉瘤细胞中，miR-21 可抑制 PTEN 的表达，激活 MMP-2 和 MMP-9 信号通路，促进主动脉瘤细胞的增殖和迁移。
