Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6949-6954. doi: 10.26355/eurrev_202006_21686.
The purpose of this study was to explore the changes in the expressions of micro ribonucleic acid (miR)-22-3p and matrix metalloproteinase-9 (MMP-9) in rats with thoracic aortic aneurysm (TAA) and their significance.
A total of 16 specific pathogen-free Sprague-Dawley female rats were randomly divided into normal group (n=8) and angiotensin II (Ang II) group (n=8). Ang II was perfused using the micro pump in Ang II group, while the same amount of normal saline was perfused in the normal group. After continuous intervention, the tumor formation rate in the thoracic aorta was observed, and the expression of miR-22-3p was detected via Reverse Transcription-Polymerase Chain Reaction (RT-PCR) in both groups. Other 16 rats were selected and randomly divided into agomiR-22-3p group (n=8) and control group (n=8). In the agomiR-22-3p group, agomiR-22 and Ang II were continuously injected via angular vein. In the control group, agomiR negative control was injected, and Ang II was continuously perfused. After intervention for 4 weeks, the tumor formation rate in the thoracic aorta was observed, and the expression of MMP-9 was determined via immunofluorescence and immunohistochemistry in both groups.
After intervention for 4 weeks, the expression of miR-22-3p in Ang II group was significantly lower than that in normal group (p<0.05). After drug administration for 4 weeks, agomiR-22-3p group had a lower tumor formation rate (p<0.05) and a lower expression of MMP-9 than the control group (p<0.05).
The expression of miR-22-3p declines in TAA rats, and miR-22-3p can inhibit the expression of MMP-9, thus suppressing the formation of TAA in rats.
本研究旨在探讨微小核糖核酸（miR）-22-3p 和基质金属蛋白酶-9（MMP-9）在胸主动脉瘤（TAA）大鼠中的表达变化及其意义。
将 16 只无特定病原体的 Sprague-Dawley 雌性大鼠随机分为正常组（n=8）和血管紧张素 II（Ang II）组（n=8）。Ang II 组通过微泵灌注 Ang II，正常组则灌注等量的生理盐水。连续干预后，观察各组大鼠胸主动脉瘤的形成率，并采用逆转录聚合酶链反应（RT-PCR）检测两组大鼠 miR-22-3p 的表达情况。另选取 16 只大鼠，随机分为 agomiR-22-3p 组（n=8）和对照组（n=8）。agomiR-22-3p 组经尾静脉持续注射 agomiR-22 和 Ang II，对照组则注射 agomiR 阴性对照和 Ang II。连续干预 4 周后，观察各组大鼠胸主动脉瘤的形成率，并采用免疫荧光和免疫组化法检测两组大鼠 MMP-9 的表达情况。
干预 4 周后，Ang II 组大鼠 miR-22-3p 的表达明显低于正常组（p<0.05）。药物干预 4 周后，agomiR-22-3p 组大鼠的胸主动脉瘤形成率（p<0.05）和 MMP-9 表达水平（p<0.05）均低于对照组。
TAA 大鼠 miR-22-3p 的表达下调，miR-22-3p 可抑制 MMP-9 的表达，从而抑制大鼠 TAA 的形成。
