State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
J Med Food. 2020 Feb;23(2):191-197. doi: 10.1089/jmf.2019.4423.
was recorded in the Chinese Pharmacopoeia and traditional Chinese medical books for chronic cough, but the effect and related constituents are still unknown. Thus, we investigated the protect effects and the relevant constituents of in a guinea pig model with chronic cough induced by cigarette smoke (CS). The organic acids and polysaccharides in were detected by high performance liquid chromatography, gel permeation chromatography, and gas chromatography-mass spectrometry. The guinea pigs were orally administrated with vehicle or the water extract of (FW) during the 14 days of CS exposure. Citric acid induced coughs were automatically measured by Buxco system. The differential cells in bronchoalveolar lavage fluid (BALF) and histopathological changes in lung tissue were assessed by hematoxylin and eosin staining. The tumor necrosis factor alpha (TNF-) and interleukin-8 (IL-8) levels in lung tissue were detected via enzyme-linked immunosorbent assay. The mucus productions in tracheas were determined with Alcian blue-periodic acid Schiff staining. The results suggested relatively high concentration of citric acid, chlorogenic acid, and neochlorogenic acid in , and high proportion of galactose and glucose and lower molecular weight of polysaccharides. Administration of FW significantly reduced the cough frequency, decreased inflammatory cells in BALF and lung tissue, and attenuated the thickening of airway epithelium and submucosa compared with CS-exposure group. Moreover, the overproduction of TNF- and IL-8 in lung tissues, and mucus in central airways of CS-induced guinea pigs was markedly inhibited by FW. The extract could also protect against CS exposure-induced chronic cough in guinea pigs by reducing coughs, airways inflammation, and mucus overproduction.
在中国药典和中医古籍中被记载用于治疗慢性咳嗽，但作用和相关成分仍不清楚。因此，我们研究了 对香烟烟雾（CS）诱导的慢性咳嗽豚鼠模型的保护作用及相关成分。采用高效液相色谱法、凝胶渗透色谱法和气相色谱-质谱法检测 的有机酸和多糖。在 CS 暴露的 14 天内，豚鼠经口给予溶剂或 的水提取物（FW）。Buxco 系统自动测量枸橼酸诱导的咳嗽。苏木精和伊红染色评估支气管肺泡灌洗液（BALF）中的差异细胞和肺组织的组织病理学变化。酶联免疫吸附试验检测肺组织中肿瘤坏死因子-α（TNF-α）和白细胞介素-8（IL-8）水平。用阿利新蓝-过碘酸希夫染色测定气管中的粘液产生。结果表明， 中柠檬酸、绿原酸和新绿原酸的浓度相对较高，半乳糖和葡萄糖的比例较高，多糖的分子量较低。与 CS 暴露组相比，FW 给药显著降低了咳嗽频率，减少了 BALF 和肺组织中的炎症细胞，并减轻了气道上皮和粘膜下的增厚。此外，FW 还明显抑制了 CS 诱导的豚鼠肺组织中 TNF-α和 IL-8 的过度产生，以及中央气道中的粘液过度产生。该提取物还可以通过减少咳嗽、气道炎症和粘液过度产生来预防 CS 暴露引起的慢性咳嗽。
