SPO, Univ Montpellier, UMR INRAE Supagro Université de Montpellier, Faculté de pharmacie, 15 Avenue Charles Flahault, CC 800 BP 14491, 34093 Montpellier Cedex 5, France.
IRMB, Univ Montpellier, INSERM, CHU Montpellier, CNRS, Montpellier, France.
Food Chem. 2020 Jun 15;315:126233. doi: 10.1016/j.foodchem.2020.126233. Epub 2020 Jan 20.
Botrytized sweet wines are made with berries infected by the fungus Botrytis cinerea. The aim of this study was to identify biomarkers of B. cinerea infection in sweet wines with a focus on laccases which are exocellular oxidase enzymes produced by this fungus during fruit contamination. Total proteins from six commercial sweet wines, including three naturally botrytized wines and three non-botrytized wines were analysed by LC-QTOF-MS. Five laccases, namely laccase-1-BcLCC1, laccase-2-BcLCC2, laccase-3-BcLCC7, laccase-8-BcLCC8 and laccase-12-BcLCC12, were identified in both types of wine. Then, a targeted proteomic approach by LC-MRM was used to semi-quantify laccase-2-BcLCC2 and laccase-3-BcLCC7, in the six samples. LC-MRM targeted analysis of the two enzymes allowed the discrimination of botrytized versus non-botrytized sweet white wines.
贵腐甜酒是用感染了葡萄孢菌（Botrytis cinerea）的浆果制成的。本研究旨在鉴定甜酒中葡萄孢菌感染的生物标志物，重点是漆酶，这是该真菌在果实污染过程中产生的细胞外氧化酶。使用 LC-QTOF-MS 对来自六种商业甜酒的总蛋白进行分析，其中包括三种天然贵腐甜酒和三种非贵腐甜酒。在两种类型的酒中均鉴定出 5 种漆酶，分别为漆酶-1-BcLCC1、漆酶-2-BcLCC2、漆酶-3-BcLCC7、漆酶-8-BcLCC8 和漆酶-12-BcLCC12。然后，使用 LC-MRM 进行靶向蛋白质组学分析，对半定量分析这六种样品中的漆酶-2-BcLCC2 和漆酶-3-BcLCC7。LC-MRM 对这两种酶的靶向分析能够区分贵腐甜白葡萄酒和非贵腐甜白葡萄酒。
