Backmann Louis, Umberath Kim Marie, Wegmann-Herr Pascal, Weber Fabian, Jürgens Andreas, Scharfenberger-Schmeer Maren
Department of Biology, Chemical Plant Ecology, Technische Universität Darmstadt, Schnittspahnstrasse 4, D-64287 Darmstadt, Germany.
Institute for Viticulture and Oenology, Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Breitenweg 71, D-67435 Neustadt, Germany.
Microorganisms. 2025 Feb 21;13(3):483. doi: 10.3390/microorganisms13030483.
One of the most important crop pathogens is . It overcomes plant defenses using laccase, an enzyme which is frequently researched. Yet the differences between strains regarding their laccase activity is poorly understood. The aim of this study was to analyze laccase genes in the context of the regionality, vintage, and laccase activity of the strains. Eight strains were analyzed using whole genome sequencing, and the laccase activity was assessed. The strains were differentiated by SSR-PCR. We looked at all 14 known laccase genome regions as well as the promoter and terminator regions using variant metrics and phylogenetic trees. The laccase genes seem to be correlated with the regionality of the strains rather than the laccase activity, which provides new understanding to the study of pathogen adaption in specific environments. Some of the laccase gene regions showed little to no evolutionary change, while other regions showed a great variety of changes. This research highlights taking different laccase gene regions into context. We provide fundamental information for further research. Further studies, especially on gene expression, could provide insightful information regarding the potential of pathogen infection.
最重要的作物病原体之一是……它利用漆酶克服植物防御，漆酶是一种经常被研究的酶。然而，关于菌株之间漆酶活性的差异却知之甚少。本研究的目的是在菌株的区域性、年份和漆酶活性的背景下分析漆酶基因。使用全基因组测序分析了八个菌株，并评估了漆酶活性。通过SSR-PCR对菌株进行区分。我们使用变异指标和系统发育树研究了所有14个已知的漆酶基因组区域以及启动子和终止子区域。漆酶基因似乎与菌株的区域性相关，而非与漆酶活性相关，这为特定环境中病原体适应性的研究提供了新的认识。一些漆酶基因区域几乎没有进化变化，而其他区域则显示出各种各样的变化。本研究强调将不同的漆酶基因区域纳入背景考量。我们为进一步研究提供了基础信息。进一步的研究，特别是关于基因表达的研究，可能会提供有关病原体感染潜力的有见地的信息。
